Dimethyl sulphate

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Reliable well documented study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Transplacental genetic and cytogenetic effects of alkylating agents in the mouse I: Induction of somatic coat color mutations

Author:

Braun R et al.

Year:

1984

Bibliographic source:

Terat Carcinog Mutagen 4:449-457

Reference 2

Reference Type:

secondary source

Title:

EU Risk Assessment Report Dimethyl Sulphate

Author:

European Chemicals Bureu

Year:

2002

Bibliographic source:

EU RAR, Volume 12, Luxembourg 2002

Materials and methods

Type of study / information:

Mouse spot test

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: OECD 484

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl sulphate

EC Number:

201-058-1

EC Name:

Dimethyl sulphate

Cas Number:

77-78-1

Molecular formula:

C2H6O4S

IUPAC Name:

dimethyl sulfate

Results and discussion

Any other information on results incl. tables

DMS did not induce RS frequency in both crosses.

Table 1: Frequency of RS, WMVS and MDS in offsring derived from the cross NMRI x DBA

Compound	Dose (mg/kg)	Treatment day	No. of females treated	No. of litters	No. of offspring^a	Average litter size^a	Offspring with			P
							RS (%)	WMVS (%)	MDS (%)
DMS	50	10.25	60	42	387 (201)	9.2 (4.7)	0 (0)	0 (0)	0 (0)	> 0.05

a: These columns refer to the total number of offspring while the number of non-agouti offspring is given in brackets.

Table 2: Frequency of RS, WMVS and MDS in offspring derived from the cross C57 x T-stock at day 10.25 of embryonal development

Compound	Dose (mg/kg)	No. of females treated	No. of litters	No. of offspring	Average litter size	Offspring with			P
						RS (%)	WMVS (%)	MDS (%)
DMS	25	87	28	181	6.5	2 (1.10)	2 (1.10)	0 (0)	> 0.05

DMS did not induce mutations up to the highest concentration.