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Toxicity to aquatic algae and cyanobacteria

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Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
09 Jan - 12 Jan 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution of 2.27 g test substance/L in VE-Water
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: 86.81 SAG
- Source: originally obtained from Insitut für Wasser-, Boden- und Lufthygiene, Berlin, Germany, since then in-house culture
- Age of inoculum (at test initiation): 3 days
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
not relevant
Hardness:
not reported
Test temperature:
22.6 - 22.9 °C
pH:
8.5 - 8.8 (measured at test start)
8.2 - 9.6 (measured at test end)
Dissolved oxygen:
not reported
Salinity:
not reported
Nominal and measured concentrations:
nominal: 0, 60, 100, 170, 300, 500 and 900 mg/L
measured geometric mean: 0, 34, 61, 103, 188, 297 and 511 mg/L
Concentration are given for anionic active substance.
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Initial cells density: 2 * 10E4 cells/mL
- Control end cells density: 88 * 10E4 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8

GROWTH MEDIUM
- Standard medium used: yes, according to EU C.3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: VE water

OTHER TEST CONDITIONS
- Light intensity and quality: 8000 Lux (white)

EFFECT PARAMETERS MEASURED: number of cells after 0, 24, 48 and 72 h, cells per mL is calculated
- Determination of cell concentrations: absorption at 685 nm with photometer

Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 511 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active substance
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
199 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active substance
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
103 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active substance
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
133 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active substance
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
511 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active substance
Basis for effect:
biomass
Details on results:
- Exponential growth in the control (for algal test): yes
Results with reference substance (positive control):
not reported
Reported statistics and error estimates:
- The NOEC was determined via t-test.
- EC50 calculation was conducted according to Probit analysis adapted from Cavalli-Sforza (1972)
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
96 h growth inhibition study on green algae
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): Norwegian Institute of Water Research (NIVA)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
4.1 mg CaCO3/L
Test temperature:
20 °C
pH:
7.0 - 7.5
Salinity:
not relevant
Details on test conditions:
TEST SYSTEM
- Test vessel: 1L flasks
- Type (delete if not applicable): open
- Aeration: 0.8% CO2 in air at a flow rate of 0.5L/min
- Initial cells density: 10000 - 11000 cells/mL

TEST MEDIUM
- Deionized water containing 0.37 mg/L Na2EDTA.2H2O, 2.1 mg/L Na2CO3

OTHER TEST CONDITIONS
- Light intensity and quality: 15000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: optical density at 600 nm
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
117 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
96 h
Dose descriptor:
EC90
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
- Exponential growth in the control (for algal test): yes, growth rate 1.23 ± 0.006/day (growth inhibition)

Reported statistics and error estimates:
Toxic responses were quantified by a simple graphical method. Relative response (absolute response devided by average response of the controls) was plotted against log concentration and EC10, EC50 and EC90 read from graph.
Growth inhibition test: Algae were grown in approximately one-liter flasks submerged in temperature-controlled aquaria. Flasks were aerated continuously with 0.8% CO2 in air at a flow rate of 0.5 l/minute. pH was adjusted daily and did not exceed 8.0. Biomass was measured once daily. Six concentrations were tested.
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
12 September - 15 September 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 9
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by dissolving 0.5001 g/500 mL (medium not defined). From stock solution the required volumina for final test concentrations were taken.
- Differential loading: yes
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: Scenedesmus subspicatus, Chlorococcales
- Strain: SAG 86.81
- Source (laboratory, culture collection): Pflanzenphysiologisches Institut Universität Göttingen

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Hardness:
no data
Test temperature:
22.5-24.5 °C
pH:
6.5 - 9.7
Nominal and measured concentrations:
Nominal: 0, 15, 30, 60 and 120 mg/L
Measured based on AS at test start: -, 14.7, 31.1, 62.8, 123.6 mg/L
Measured based on AS at test end: -, 15.7, 32, 59, 117.8 mg/L
Measured based on MBAS at test start: -, 17.8, 37.6, 75.9, 149.3 mg/L
Measured based on MBAS at test end: -, 19, 38.7, 71.3, 142.4 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flask, sterilised at 180°C
- Filling volume: 100 mL test volume
- Renewal rate of test solution (frequency/flow rate): static test
- Initial cells density: 10,000 cells/mL
- Control end cells density: 2,000,000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes (DIN 38412, part 9)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterilised, distilled water

OTHER TEST CONDITIONS
- Photoperiod: continuous ilumination
- Light intensity and quality: 2000 Lux (Li-Cor-Li-185 B)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter counter

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 fold
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
53 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
Reported statistics and error estimates:
tabular calculation method using Lotus 1-2-3 software package
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
07 Sep - 11 Sep 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 9
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution of 10 g in Aqua bidest.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: SAG 8681
- Source (laboratory, culture collection): Institute of Plant Physiology, University of Göttingen, Germany
- Age of inoculum (at test initiation): 3 days


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
no data
Test temperature:
23-23.5 °C
pH:
no data
Dissolved oxygen:
no data
Salinity:
not relevant
Nominal and measured concentrations:
Nominal: 0, 1, 10 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flask
- Test volume: 100 mL
- Initial cells density: 10000 cells/mL
- Control end cells density: 1,500,000 cells/mL (exponential growth)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


GROWTH MEDIUM
- Standard medium used: yes (Medium Algen n10.10)


OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity and quality: 2000 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter counting chambers
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
EC0
Effect conc.:
0.9 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Key result
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
7.4 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
38 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Details on results:
- Exponential growth in the control (for algal test): yes
Reported statistics and error estimates:
Probit analysis
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
25 Feb - 28 Feb 1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0, 1, 3, 10, 30, 100 mg/L
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.54 g/L test substance in VE water (stock solution)
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: CHODAT (86.81 SAG)
- Source (laboratory, culture collection): Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany (originally); in-house culture
- Age of inoculum (at test initiation): 3 days
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
not relevant
Hardness:
no data
Test temperature:
23.5-23.8 °C
pH:
Begin: 7.8-8.0
End: 7.3-9.4
Dissolved oxygen:
no data
Salinity:
not relevant
Nominal and measured concentrations:
Nominal: 0, 1.5, 3, 6, 12, 24, 48 and 96 mg/L
Measured: >80% at all concentrations, nominal values used
Details on test conditions:
TEST SYSTEM
- Test vessel: sterile Erlenmeyer flasks
- Initial cell density: 20000 cells/mL
- Control end cells density: 1,160,000 cells/mL

GROWTH MEDIUM
- Standard medium used: yes


OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous illumination
- Light intensity and quality: 8000 lux (white)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cell number and cell growth
- Determination of cell concentrations: spectrophotometer at 685 nm


Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
7.6 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
6.4 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
biomass
Details on results:
- Exponential growth in the control (for algal test): yes (20,000 to 1,160,000 cells/mL)
- Observation of abnormalities (for algal test): no
Reported statistics and error estimates:
Probit analyis (Cavalli-Sforza 1972)
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
28 Jul - 31 Jul 1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: DIN 38412 part 9; German standard method (group L); determination of the inhibitory effect of water constituents on green algae (Algae growth inhibition test), L9
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
25 - 26 °C
pH:
6.3 - 8.9
Nominal and measured concentrations:
nominal: 0, 0.03, 0.1, 0.3, 1.0, 3.0, 10, 30, 100, 300, 1000, 3000 mg/L
Reference substance (positive control):
no
Duration:
72 h
Dose descriptor:
EC0
Effect conc.:
17 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
biomass
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
34 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth rate
Details on results:
EC10 biomass = ca. 19 mg a.i./L
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
30 Jan -02 Feb 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.68 g test substance were dissolved in 1L VE-water
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: CHODAT 86.81 SAG
- Source (laboratory, culture collection): Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age of inoculum (at test initiation): 3 days
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
not applicable
Test temperature:
22.1-22.3 °C
pH:
Begin: 8.2-8.3
End: 7.8-9.4
Nominal and measured concentrations:
Nominal: 0, 0.6, 1.25, 2.5, 5, 10 and 20 mg/L (concentration of active ingredient)
Measured: 0.12, 0.65, 1.2, 2.3, 4.6, 9.7 and 20 mg/L (0 hours, concentration of active ingredient)
Measured: 0.0, 0.61, 0.87, 1.8, 3.4, 12 and 17 mg/L (72 hours, concentration of active ingredient)
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Aeration: sterile aeration
- Renewal rate of test solution (frequency/flow rate): none
- Initial cells density: 20,000 cells/mL
- Control end cells density: 1,040,000 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5


OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24 hours
- Light intensity and quality: 8000 Lux, white


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: with spectrophotometer at at 685 nm


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 20 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
5.4 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.6 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
2 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
biomass
Details on results:
- Exponential growth in the control (for algal test): yes
Reported statistics and error estimates:
Probit analysis according to Cavalli-Sforza (1972)
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
02 May - 05 May 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: all concentrations except stock solution
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 2.55 g test material/L VE-water (corresponds to 1 g a-WAS/L)
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: 86.81 SAG
- Source : in-house culture, originally obtained from Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age of inoculum: 3 days


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
not relevant
Test temperature:
24.0 - 24.2 °C
pH:
Test start: 7.2-8.1
Test end: 7.7-9.4
Nominal and measured concentrations:
Nominal: 0, 1.2, 3.0, 7.5, 20, 50, 125 and 300 mg/L
Measured (geometrical mean) values have a deviation < 20% and nominal values were used
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Aeration: sterile aeration
- Initial cells density: 2*10E4 cells/mL
- Control end cells density: 106000 cells/mL (approximation - read from graph)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24 hours light
- Light intensity and quality: 8000 lux

EFFECT PARAMETERS MEASURED: cell growth and growth rate after 0, 24, 48 and 72 h
- Determination of cell concentrations: spectrophotometer, 685 nm


Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
2.7 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
9.3 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
biomass
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
11 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Details on results:
- Exponential growth in the control: yes

Reported statistics and error estimates:
Determination of EC values: probit transformation with procentual inhibition values and regression analysis
Probit analysis according to Cavalli-Sforza (1972).

At the highest test concentration the turbidity falsified the results. Thus, it was not included in the evaluation.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Justification for type of information:
Read across justification document is attached in chapter 13
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
34 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth rate
Remarks on result:
other: CAS 68955-20-4
Duration:
96 h
Dose descriptor:
EC0
Effect conc.:
0.9 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Remarks on result:
other: CAS 68955-19-1
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
7.4 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Remarks on result:
other: CAS 68955-19-1
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
38 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Remarks on result:
other: CAS 68955-19-1
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Remarks on result:
other: CAS 68955-19-1
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
7.6 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Remarks on result:
other: CAS 68955-19-1
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Remarks on result:
other: CAS 68955-19-1
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.6 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Remarks on result:
other: CAS 85586-07-8
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
5.4 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Remarks on result:
other: CAS 85586-07-8
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 20 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Remarks on result:
other: CAS 85586-07-8
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
11 mg/L
Nominal / measured:
nominal
Conc. based on:
other: a-WAS
Basis for effect:
growth rate
Remarks on result:
other: CAS 90583-18-9
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: CAS 151-21-3
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
117 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: CAS 151-21-3
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: CAS 151-21-3
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
199 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active substance
Basis for effect:
growth rate
Remarks on result:
other: CAS 126-92-1
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 511 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
act. ingr.
Remarks:
anionic active substance
Basis for effect:
growth rate
Remarks on result:
other: CAS 126-92-1

Description of key information

EC50(72h)= 11 mg/L (nominal)for growth rate of Desmodesmus subspicatus (EU Method C.3, read across)
NOEC(72h) = 0.6 mg/L (nominal) for growth rate of Desmodesmus subspicatus (EU Method C.3, read across)

 


 

Key value for chemical safety assessment

EC50 for freshwater algae:
11 mg/L
EC10 or NOEC for freshwater algae:
0.6 mg/L

Additional information

Since no reliable aquatic algae test is available for C8-18AS Mg & TEA (CAS 85586-38-5), in accordance to Regulation (EC) 1907/2006 Annex XI, 1.5, a read-across to another structurally related alkyl sulfates was conducted. Read-across data from the following substances were used: with, C8-isoAS Na (CAS 126-92-1), C12AS Na (CAS 151-21-3), C12-14AS TEA (CAS 90583-18-9), C12-14AS Na (CAS 85586-07-8), C12-18AS Na (CAS 68955-19-1) and C16-18AS Na (CAS 68955-20-4). Toxicity of alkyl sulfates is dependent on the carbon chain length and the influence of the counter ion can be neglected.


One study investigating toxic effects of C8-isoAS Na (CAS 126-92-1) to freshwater algae Desmodesmus subspicatus is available (Scholz 1995). The test was performed according to EU Method C.3 and is GLP compliant. Growth inhibition caused by the test substance was determined after 24, 48 and 72 h of exposure to five test concentrations up to 511 mg a.i./L (measured concentration equal to 900 mg a.i./L of nominal concentration). The results show that the 72-hour EC50 value for growth rate was above the highest test concentration of 511 mg a.i./L (measured concentration). The 72-hour EC10 value for growth rate was determined to be 199 mg a.i./L (measured concentration).


Moreover, a GLP compliant study was performed with C12AS Na (CAS 151-2-3) according to DIN 38412, part 9 guideline (Rieche 1994). Aquatic algae (Desmodesmus subspicatus) were exposed for 72 hours to four test concentrations up to 120 mg/L as nominal concentrations. Analytical check of test concentrations showed that the nominal concentrations were in agreement with the measured ones and therefore the nominal concentrations were used. The 72-h EC50 value for growth rate was above 120 mg/L (nominal concentration). The NOEC value for biomass was 30 mg/L (nominal concentration). These results are supported by a publication of Nyholm and Damgaard (1990). The non-GLP, test was performed with Pseudokirchnerella subcapitata exposed to six test concentrations. No special guideline was followed and analytical monitoring of test concentrations was not performed. Growth rate measurement after 96-h resulted in the ErC50 value of 117 mg/L (nominal concentration). The NOEC value for growth rate was 12 mg/L (nominal concentration).


The study investigating toxic effects of C12-14AS TEA (CAS 90583-18-9) to freshwater algae Desmodesmus subspicatus was performed according to EU Method C.3 and GLP principles (Scholz 1996). Growth inhibition caused by the test substance was determined after 24, 48 and 72 h of exposure under static conditions to seven test concentrations up to 300 mg a.i./L (nominal values in agreement with the measured values). The results show that the 72-hour EC50 value for growth rate was 11 mg a.i./L for growth rate and 9.3 mg a.i./L for biomass (nominal concentrations). The NOEC value for growth rate was determined to be 3 mg a.i./L (nominal concentration).


Additionally, a supporting GLP-compliant study with Desmodesmus subspicatus is available for C12-14AS Na (CAS 85586-07-8, Scholz 1995). This test was performed according to EU Method C.3. Algae were exposed to six test concentrations up to 20 mg/L (nominal concentration corresponding to 17 mg/L of measured concentration). The study resulted in the 72-hour EC50 value for growth rate of above 20 mg a.i./L (nominal concentration). The EC10 (72 h) for growth rate was 5.4 mg a.i./L (nominal concentration).


Two GLP compliant studies are available for C12-18AS Na (CAS 68955-19-1). The first study was performed with C12-18AS Na (CAS 68955-19-1) according to the EU method C.3 (Scholz 1997). Freshwater algae (Desmodesmus subspicatus) were exposed for 72 hours to seven test concentrations up to 96 mg/L (nominal concentrations in agreement with the measured ones). The results showed that the 72-hour EC50 value for growth rate was 20 mg a.i./L and the EC10 value for growth rate was 7.6 mg/L. The second study with C12-18AS Na (CAS 68955-19-1) was conducted according to the German standard method DIN 38412, part 9 (Mühlberg 1992). Desmodesmus subspicatus were exposed to for 96 hours to the nominal concentrations up to 100 mg/L, no analytical measurements are available. The reported EC50 value was 38 mg/L based on cell number. The EC10 value reached 7.4 mg/L.


Moreover, one study investigating toxic effects of C16-18AS Na (CAS 68955-20-4) to freshwater algae is available. The non-GLP study was performed with Desmodesmus subspicatus according to EU Method C.3 (Richterich 2001). Growth inhibition caused by the test substance was determined after 24, 48 and 72 hours of exposure to eleven test concentrations up to 3000 mg/L substance of 55.5% purity (equal to 1650 mg a.i./L). No analytical monitoring is available. The results show that the 72-hour EC50 value for growth rate was 34 mg a.i./L and for biomass 30 mg a.i./L (nominal concentrations). The EC10 for biomass was 19 mg a.i./L (nominal concentrations).


Due to structural and property similarities these results apply also to C8-18AS Mg & TEA (CAS 85586-38-5). The composition of C8-18AS Mg & TEA (CAS 85586-38-5) is C8: 15.8%, C10: 15.8%, C12: 44.7%, C14: 15.8%, C16: 5.3% and C18: 2.6%. The EC50 and NOEC values of 11 mg/L and 0.6 mg/L respectively (both for growth rate) obtained in the aquatic algae test with C12-14AS TEA (CAS 90583-18-9) and C12-14AS Na (CAS 85586-07-8) are the most conservative values and they considered to be a reliable also for C8-18AS Mg & TEA (CAS 85586-38-5).