Sulfuric acid, mono-C8-18-alkyl esters, magnesium salts, compds. with triethanolamine

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

fish, juvenile growth test

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03 Sept - 14 Oct 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

42 day exposure of fish in aquaria provided with continuous flow of river water (no cycle), stock solution addition.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Exposure concentrations were maintained by amending river water at the head of each stream with surfactant stocks in a dilution series, stocks were prepared in de-ionized carbon filtered water

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnow
- Source: Virginia Tech, Department of Biology, Virginia
- Age at study initiation (mean and range, SD): 4 weeks post hatch
- Feeding during test: provided by stream water
- Food type: natural organisms/detritus

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

42 d

Post exposure observation period:

not relevant

Test temperature:

Test begin: approx. 24 °C
Test end: approx. 13 °C

Salinity:

not relevant

Nominal and measured concentrations:

Nominal: 0, 26, 78, 233, 700 and 2100 µg/L
Measured: 2 ± 5, 23 ± 30, 51 ± 17, 151 ± 44, 418 ± 103 and 1357 ± 408 µg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Flow through aquaria drawing water from experimental stream channels
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 75 L
- Type of flow-through (e.g. peristaltic or proportional diluter): peristaltic pump
- Renewal rate of test solution (frequency/flow rate): 400 mL/min (hydraulic residence time was 160 minutes)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : juvenile mortality and juvenile growth

Reference substance (positive control):

no

Key result

Duration:

42 d

Dose descriptor:

NOEC

Effect conc.:

>= 1.357 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

weight

Remarks on result:

other: ± 0.408 mg/L

Key result

Duration:

42 d

Dose descriptor:

NOEC

Effect conc.:

>= 1.357 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: ± 0.408 mg/L

Reported statistics and error estimates:

Parametric and non-parametric analysis of variance (Kruskal-Wallis), using SAS (Statistical Analysis System 1990). Multiple range tests used were at Least Significant Differences Procedure and Wilcoxon Rank Sum. NOEC are defined as the highest exposure concentration which did not significantly impair the tested population relative to the control.

Validity criteria fulfilled:

not applicable

Reference 2

Endpoint:

fish, juvenile growth test

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Read across justification is attached in chapter 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

42 h

Dose descriptor:

NOEC

Effect conc.:

>= 1.357 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

other: mortality and weight

Description of key information

 NOEC(42d)>= 1.357 mg/L(measured) for motality and growth of Pimephales promelas (read across)

Additional information

Since no reliable chronic fish test is available for C8-18AS Mg & TEA (CAS 85586-38-5), in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5, a read-across to the main constituent C12AS Na (CAS 151-21-3) was used. The read across justification is attached in chapter 13.

A 42-day flow-through study with fathead minnow (Pimephales promelas) examining the toxic effect of CAS 151-21-3 is available (Belanger et al. 1995). Juvenile mortality and juvenile growth were determined in a non-GLP test after exposure to five concentrations up to 1.357 mg/L (measured concentration, equal to 2.1 mg/L of nominal concentration). Since no adverse effects were observed regarding fish survival and body weight, the NOEC value was determined as equal as or higher than 1.357 mg/L (measured concentration). Due to structural and property similarities with the source substance it can be concluded that the above results apply also to C8-18AS Mg & TEA (CAS 85586-38-5).