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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Batch no.: 0016420429
- Purity: ca. 76 %
- Impurities: 17.9 % triethanolamine, 2.5 % sulfates, 2.5% Mg, 1.6% water, 0.05 % cyclohexanol

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
The stability of the test substance under storage conditions
over the test period was guaranteed by the sponsor, and the
sponsor holds this responsibility
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Source: Activated sludge from the wastewater treatment plant of Lambsheim, Germany
Concentration of dry substance: 30 mg/L
Duration of test (contact time):
28 d
Initial conc.:
40 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
TOC
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance:
aniline
Preliminary study:
Determination of total organic carbon by combustion
Key result
Parameter:
% degradation (CO2 evolution)
Value:
82
Sampling time:
28 d
Details on results:
10% biodegradation degree after ca. 1 day. Degree of biodegradation at the end of the ten-day window ca. 68%
Results with reference substance:
Degree of biodegradation after 14 days: 77%
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable

Description of key information

Readily biodegradable (according to OECD criteria): 82% in 28 days, 68% within the 10-day window (OECD 301B)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information

In a CO2-Evolution test the biodegradation of the test item was investigated according to OECD guideline 301B (BASF 2019). After 28 days a biodegradation of 82% was observed. At the end of the ten day window the degree of biodegradation was ca. 68%. Therefore, the required pass level for ready biodegradability i.e., at least 60% of the theoretical CO2 production in a 10-day window exceeding 10% degradation within the 28-day period of the test was reached.