Sulfuric acid, mono-C8-18-alkyl esters, magnesium salts, compds. with triethanolamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch no.: 0016420429
- Purity: ca. 76 %
- Impurities: 17.9 % triethanolamine, 2.5 % sulfates, 2.5% Mg, 1.6% water, 0.05 % cyclohexanol

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
The stability of the test substance under storage conditions
over the test period was guaranteed by the sponsor, and the
sponsor holds this responsibility

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Source: Activated sludge from the wastewater treatment plant of Lambsheim, Germany
Concentration of dry substance: 30 mg/L

Duration of test (contact time):

28 d

Initial conc.:

40 mg/L

Based on:

test mat.

Initial conc.:

20 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

aniline

Preliminary study:

Determination of total organic carbon by combustion

Key result

Parameter:

% degradation (CO2 evolution)

Value:

82

Sampling time:

28 d

Details on results:

10% biodegradation degree after ca. 1 day. Degree of biodegradation at the end of the ten-day window ca. 68%

Results with reference substance:

Degree of biodegradation after 14 days: 77%

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Description of key information

Readily biodegradable (according to OECD criteria): 82% in 28 days, 68% within the 10-day window (OECD 301B)

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

In a CO₂-Evolution test the biodegradation of the test item was investigated according to OECD guideline 301B (BASF 2019). After 28 days a biodegradation of 82% was observed. At the end of the ten day window the degree of biodegradation was ca. 68%. Therefore, the required pass level for ready biodegradability i.e., at least 60% of the theoretical CO2 production in a 10-day window exceeding 10% degradation within the 28-day period of the test was reached.