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Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From Aug. 30, 2010 to Sept. 08, 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
yes
Remarks:
The relative standard deviation of six replicates exceeds the limit (≤15%), but the TOC analysis was considered as acceptable (see 'Any other information on results incl. tables' for further details)
Principles of method if other than guideline:
Not applicable
GLP compliance:
not specified
Type of method:
flask method
Water solubility:
36 mg/L
Temp.:
20 °C
pH:
7.9 - 8.4
Details on results:
- Water solubility of the test substance calculated via total organic carbon (TOC) at 20˚C is 36 mg/L.
- pH value of the sample solution was 7.9, 8.1, and 8.4 after 24, 48 and 72 h of incubation respectively.

Table 1: Water solubility study results

The concentrations of the different test solutions were calculated automatically using the equation of the calibration curve and considering the dilution factors of the test solutions. The results are listed in the following table (all results given in mg/L):

Incubation time (hours)

 

Water solubility (TOC mg/L)

 

Total Average (TOC mg/L)

 

24

35

36

48

42

72

30

* standard deviation: 6.0 mg/L; relative standard deviation: 17.0%

- The relative standard deviation of six replicates exceeds the limit (≤15%) according to the test procedure. However, as the test substance was identified as a heterogenous mixture (with wide range of polarities) as per the GCMS chromatogram, a more accurate determination was not considered possible. Hence, the TOC analysis was considered as the appropriate technique to quantify the analyte in aqueous solution, and the relative standard deviation of 17% was considered to be acceptable.

Conclusions:
Interpretation of results (migrated information): slightly soluble (0.1-100 mg/L)
Under the test conditions, the water solubility of 'amides, C12-18(even-numbered) and C18(unsatd.), N-(hydroxyethyl)' was determined to be 36 mg/L at 20 °C (i.e., slightly soluble).
Executive summary:

A study was performed to determine the water solubility of ‘amides, C12-18(even-numbered) and C18(unsatd.), N-(hydroxyethyl)’ according to the EU Method A.6.

A preliminary experiment performed with the test substance showed high water solubility of above 10E-2 g/L. Hence, the water solubility was determined by the "shake flask method".

0.1773 g of the test substance (0.1561 g, 0.1197 g) was transferred to 100 mL of water and stirred in a heated water bath for 24 hours (48 hours, 72 hours) at 30˚C. After incubation, the sample suspension was cooled to 20˚C and stirred for 24 hours. The supernatant was then filtered using a syringe filter. After centrifugation (for 20 minutes, 4500 rpm) the clear supernatant was analyzed via TOC (Total organic carbon) method.

Under the test conditions, the water solubility of ‘amides, C12-18(even-numbered) and C18(unsatd.), N-(hydroxyethyl)’ was determined to be 36 mg/L at 20 °C (i.e., slightly soluble).

Description of key information

The water solubility was determined according to EU Method A.6 (Frischmann, 2010).

Further water solubility testing according to OECD Guideline 105 is planned.

Key value for chemical safety assessment

Water solubility:
36 mg/L
at the temperature of:
20 °C

Additional information