Reaction mass of 2-(methylamino)ethanol, compound with sulphur dioxide and bis[(2-hydroxyethyl)ammonium] sulphite

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given, adequate for assessment

Data source

Reference

Reference Type:

publication

Title:

Distribution and metabolism of topically applied ethanolamine

Author:

Klain GJ, et al.

Year:

1985

Bibliographic source:

Fundam. Appl. Toxicol. 5, 127 -133

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The skin resorption of ethanolamine was tested on isolated skin of pigs in vitro.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-aminoethanol

EC Number:

205-483-3

EC Name:

2-aminoethanol

Cas Number:

141-43-5

IUPAC Name:

2-aminoethanol

Details on test material:

- Name of test material (as cited in study report): ethanolamine
- Radiochemical purity (if radiolabelling): >95 %
- Specific activity (if radiolabelling): 4 mCi/mmol)
- Locations of the label (if radiolabelling): [1.2-14 C] Ethanolamine - HCI

Radiolabelling:

yes

Test animals

Species:

pig

Strain:

not specified

Sex:

not specified

Administration / exposure

Doses:

5 µl to 0.8 cm² area of pig skin, resulting in a dose of 4 µg/cm² and a radioactive dose of approximately 0.05 µCi

Results and discussion

Percutaneous absorption

Percutaneous absorption 1

Dose:

4 μg/cm2

Parameter:

percentage

Absorption:

ca. 1 %

Remarks on result:

other: 2 h

Percutaneous absorption 2

Dose:

4 μg/cm2

Parameter:

percentage

Absorption:

ca. 3 %

Remarks on result:

other: 24 h

Percutaneous absorption 3

Dose:

4 μg/cm2

Parameter:

percentage

Absorption:

ca. 5 %

Remarks on result:

other: 50 h

Any other information on results incl. tables

The in vitro data show that a relatively small percentage of the applied dose of ethanolamine was lost from the skin by evaporation and percutaneous penetration. At the end of the 50 hour test period the main bulk of the compound remained on the skin. In separate experiments 7 to 9 times as much radioactivity was recovered from the upper 100 µm layer (mostly epidermis) as compared to the recovery from the remaining dermis.

 

Disposition of ethanolamine after topical application to pig skin in vitro at a chemical dose of 4 µg/cm²

 

Time interval after application (hrs)	Evaporation	Percutaneous penetration	Skin residue
2	6 ± 7	1 ± 1	-*
24	17 ± 3	3 ± 2	-**
50	11 ± 7	5 ± 2	62 ± 18***

data presented as mean ± SD percentage of applied radioactive dose (n=3)

*not assayed

**assayed for transformation of ethanolamine into skin metabolites

***radioactivity recovered from combustion of the entire skin sample at the end of the time interval