Cetrimonium bromide

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Although no guideline was followed the study contains sufficient experimental details to be evaluated satisfactory and reliable with restrictions.

Data source

Reference

Reference Type:

publication

Title:

Embryotoxic and teratogenic effects of CTAB, a cationic surfactant, in the mouse

Author:

B. Isomaa and K. Ekman

Year:

1975

Bibliographic source:

Fd Cosmet. Toxicol. Vol 13 pp 331-334

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

CTAB in aqueous solution was given in single ip injections to four groups each of eight mice. The median lethal dose and its standard error were estimated accorting to Miller & Tainter (1994)*.
*: Miller, L.C.. & Tainter, M.L. (1994). Estimation of the ED 50 and its error by means of logarithmic-probit graph paper. Proc. Soc. exp. Biol. Med. 57, 261.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Cetyl trimethylammonium bromide (CTAB)

IUPAC Name:

Cetyl trimethylammonium bromide (CTAB)

Test material form:

not specified

Test animals

Species:

mouse

Strain:

NMRI

Sex:

female

Details on test animals or test system and environmental conditions:

The animals were given commercial pellets and water ad. lib. and maintained in a 12 hour light-dark sequence under ambient conditions of 20± 1°C and 60 ± 5% humidity.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: aqueous solution

Details on exposure:

Single ip injections

Doses:

no data

No. of animals per sex per dose:

Four groups each of eight mice

Control animals:

not specified

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

>= 85 - <= 127 mg/kg bw

Based on:

not specified

Mortality:

Death occured generally within 12 hr of administration. The animals that did not die recovered within 24 hr, although some delayed deaths did occur.

Clinical signs:

convulsions and respiratory depression

Applicant's summary and conclusion

Conclusions:

The acute ip LD50 for female mice was found to be 106±20 mg/kg bw.

Executive summary:

The acute ip LD50 for female mice was found to be 106±20 mg/kg bw.