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EC number: 701-083-2
CAS number: -
The ready biodegradability was determined with a non adapted activated
sludge for the test item CAS-NO. 68512-53-8 (batch no.: ESDB01441) over
a test period of 28 days in the Modified Sturm Test. The study was
conducted from 2002-03-14 to 2002-04-12 according to OECD 301 B/COz
evolution test in the DR.U.NOACK-LABORATORFIUOMR A NGEWANDTE BIOLOGIE.
The test item was tested in a concentration of 30 mg/L in duplicates,
corresponding to a carbon content (TOC) of 11.1 mgC/L in the test
vessels. The biodegradation of the test item was followed by titrimetric
analyses of the quantity of Cop, which was produced by the respiration
of bacteria. The degradation was finished on day 28 by acidification,
the last titration was made on day 29, after the soluble C02 was turned
out over a period of 24 h. The C02 production was calculated as the
percentage of total COZ that the test item could have theoretically
produced based on carbon content. Biodegradation is therefore expressed
as percentage ThC02 and was calculated for each titration of COZ. , In
order to check the activity of the test system sodium acetate was used
as functional control. The percentage degradation of the functional
control reached the pass level of > 60 % after 7 days. After 14 days a
degradation rate of 78 % was reached. The validity criterion of the
guideline is fulfilled. In the toxicity control containing both test and
reference item a biodegradation rate of 50 % occurred within 14 days and
came to a maximum of 68 % after 28 days. The biodegradation of the
reference item was not inhibited by the test item in the toxicity
control. The biodegradation of the test item is shown graphically in
Figure 1 in comparison to the readily degradable functional control and
the toxicity control. The 10 % level (beginning of biodegradation) was
reached after an adaptation phase of 3 days. In the 10-d-window a mean
biodegradation rate of 52 % was reached. After 14 days the
biodegradation came to 56 % and after 16 days the level of 60 % was
passed. The mean biodegradation came to a maximum of 82 % after 28 days.
The test item must be regarded to be in the 10-d-window, but after 16
days the level of a biodegradation of more than 60 % was passed. not
readily biodegradable The validity criteria according to the guideline
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