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EC number: 695-748-3
CAS number: 65286-55-7
Direct MTT Reduction
An assessment found the test item was able to directly reduce MTT.
Therefore, an additional procedure using freeze‑killed tissues was
performed. However, the results obtained showed that no interference due
to direct reduction of MTT occurred. It was therefore considered
unnecessary to use the results of the freeze‑killed tissues for
quantitative correction of results or for reporting purposes.
Assessment of color interference with the MTT
The solution containing the test item did not become colored. This was
taken to indicate the test item did not have the potential to cause
Test item, positive control item and negative control item
Mean OD562 values and viabilities for the negative control, positive
control and test item are given below:
MeanOD570of individual tissues
Mean OD570of duplicate tissues
Coefficient of Variation
Relative Mean Viability (%)
OD: Optical density
*: The mean percentage viability of the negative control
tissue is set at 100%
na: Not applicable
The relative mean viabilities of the test item treated tissues were as
3 minutes exposure: 75.7%
60 minutes exposure: 12.3%
The mean OD570 for the negative control treated tissues was 1.423
for the 3‑Minute exposure period and 1.567 for the 60‑Minute exposure
period. The negative control acceptance criteria were therefore
The relative mean tissue viability for the positive control
treated tissues was 0.9% relative to the negative control following the
60‑Minute exposure period. The positive control acceptance criterion was
In the range 20 to 100% viability the Coefficient of Variation
between the two tissue replicates of each treatment group did not exceed
30% with the exception of the test item treated 3 minute exposure group
which was 31.8% However, as the relative tissue viability of this group
was considerably higher than 50% this failure would not affect the
overall classification of corrosivity. This acceptance criterion was
therefore not fully satisfied and is reported as a deviation.
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