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EC number: 695-748-3
CAS number: 65286-55-7
Cumulative Mortality Data in the Definitive Test
Cumulative Mortality (Initial Population = 7)
A study was performed to assess the acute toxicity of the test
item to rainbow trout (Oncorhynchus mykiss). The method followed
was designed to be compatible with the OECD Guidelines for Testing of
Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as
Method C.1 of Commission Regulation (EC) No. 440/2008. Seven
fish were exposed to an aqueous solution of the test item, at a single
concentration of 100 mg/L for a period of 96 hours at a temperature of
approximately 15 °C under static test conditions. The number of
mortalities and any sub-lethal effects of exposure in each test and
control vessel were determined 1, 3 and 6 hours after the start of
exposure and then daily throughout the test until termination after
96 hours. Chemical analysis of the fresh test
preparations at 0 and 72 hours showed measured test concentrations of
100 and 102 mg/L. Analysis of the corresponding expired test media at 24
and 96 hours showed measured test concentrations of 102 and 103 mg/L
indicating that the concentration of test item in the test solutions had
been satisfactorily maintained and so the results are based on nominal
test concentrations only. Exposure of rainbow trout (Oncorhynchus
mykiss) to the test item gave LC50 values based on the nominal test
concentrations of greater than 100 mg/L. The No Observed Effect
Concentration (NOEC) was 100 mg/L. It was considered unnecessary and
unrealistic to test at concentrations in excess of 100 mg/L.
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