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EC number: 695-748-3
CAS number: 65286-55-7
Inhibition of Growth Rate and Yield in the Definitive Test
Geometric Mean Measured Test Concentration(mg/L)
Growth Rate (cells/mL/hour)
0 to 72 Hour
*In accordance with the OECD test guideline
only the mean value for yield for each test
concentration is calculated
R = Replicate
SD =Standard Deviation
- = Not applicable
[ ] = Increase in growth as compared to the
A study was performed to assess the effect of the test
item on the growth of the green alga Pseudokirchneriella
subcapitata. The method followed was designed to be
compatible with the OECD Guidelines for Testing of Chemicals
(2006) No 201, "Freshwater Alga and Cyanobacteria, Growth
Inhibition Test" referenced as Method C.3 of Commission
Regulation (EC) No 761/2009.
Analysis of the test preparations at 0 hours showed
measured test concentrations to range from 88% to 100% of
nominal. Analysis of the 3.2, 10, 32 and 100 mg/L test
preparations at 72 hours showed measured test concentrations to
be near nominal, a slight decline in measured test concentration
was observed in the 1.0 mg/L test preparation to 76% of nominal.
Given this decline in measured test concentrations it was
considered justifiable to base the results on the geometric mean
measured test concentrations in order to give a "worst case"
analysis of the data.
Based on the geometric mean concentrations, the 72-h EC50 based
on growth rate was determined to be 18 mg/L with a 95%
confidence interval ranging from 15 to 21 mg/L.
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