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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
(Q)SAR
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
1. SOFTWARE
On-Line EPI Suite™ User's Guide (v4.00 - v4.11).

2. MODEL (incl. version number)
BIOWIN v4.10.

3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
CN(C)CCOCCN(C)CCOCCN(C)C

4. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
BIOWIN estimates the probability of rapid aerobic and anaerobic biodegradation of an organic compound in the presence of mixed populations of environmental microorganisms.  BIOWIN contains seven separate models.  This version (v4.10) designates the models as follows (see also Boethling et al. 2003).

5. APPLICABILITY DOMAIN
see QPRF
6. ADEQUACY OF THE RESULT
see QPRF
Qualifier:
no guideline available
Principles of method if other than guideline:
- Software tool(s) used including version: Prediction of ready biodegradability based on EPI Suite v4.11 BIOWIN v4.10.
- Model(s) used: BIOWIN v4.10.
- Model description: see QMRF (Estimation of Aerobic Biodegradability using BIOWIN v4.10 (EPI Suite v4.11): BIOWIN1 to BIOWIN6 and Ready Biodegradability Prediction; See “On-Line BIOWIN™ User's Guide (v4.10)”)
- Justification of QSAR prediction: see QPRF
GLP compliance:
no
Parameter:
probability of ready biodegradability (QSAR/QSPR)
Remarks on result:
not readily biodegradable based on QSAR/QSPR prediction

SMILES : CN(C)CCOCCN(C)CCOCCN(C)C

CHEM   :

MOL FOR: C13 H31 N3 O2

MOL WT : 261.41

--------------------------- BIOWIN v4.10 Results ----------------------------

  Biowin1 (Linear Model Prediction)    :  Does Not Biodegrade Fast

  Biowin2 (Non-Linear Model Prediction):  Does Not Biodegrade Fast

  Biowin3 (Ultimate Biodegradation Timeframe):  Months

  Biowin4 (Primary  Biodegradation Timeframe):  Weeks-Months

  Biowin5 (MITI Linear Model Prediction)    :  Not Readily Degradable

  Biowin6 (MITI Non-Linear Model Prediction):  Not Readily Degradable

  Biowin7 (Anaerobic Model Prediction):  Does Not Biodegrade Fast

  Ready Biodegradability Prediction:  NO

------+-----+--------------------------------------------+---------+---------

TYPE | NUM |       Biowin1 FRAGMENT DESCRIPTION         |  COEFF  |  VALUE  

------+-----+--------------------------------------------+---------+---------

Frag |  2  |  Aliphatic ether  [C-O-C]                  | -0.3474 | -0.6947

Frag |  3  |  Tertiary amine                            | -0.2053 | -0.6158

MolWt|  *  |  Molecular Weight Parameter                |         | -0.1245

Const|  *  |  Equation Constant                         |         |  0.7475

============+============================================+=========+=========

  RESULT   |    Biowin1 (Linear Biodeg Probability)     |         | -0.6874

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM |       Biowin2 FRAGMENT DESCRIPTION         |  COEFF  |  VALUE  

------+-----+--------------------------------------------+---------+---------

Frag |  2  |  Aliphatic ether  [C-O-C]                  | -3.4294 | -6.8588

Frag |  3  |  Tertiary amine                            | -2.2229 | -6.6687

MolWt|  *  |  Molecular Weight Parameter                |         | -3.7120

============+============================================+=========+=========

  RESULT   |  Biowin2 (Non-Linear Biodeg Probability)   |         |  0.0000

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM |       Biowin3 FRAGMENT DESCRIPTION         |  COEFF  |  VALUE  

------+-----+--------------------------------------------+---------+---------

Frag |  2  |  Aliphatic ether  [C-O-C]                  | -0.0087 | -0.0173

Frag |  3  |  Tertiary amine                            | -0.2548 | -0.7644

MolWt|  *  |  Molecular Weight Parameter                |         | -0.5777

Const|  *  |  Equation Constant                         |         |  3.1992

============+============================================+=========+=========

  RESULT   |  Biowin3 (Survey Model - Ultimate Biodeg)  |         |  1.8397

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM |       Biowin4 FRAGMENT DESCRIPTION         |  COEFF  |  VALUE  

------+-----+--------------------------------------------+---------+---------

Frag |  2  |  Aliphatic ether  [C-O-C]                  | -0.0097 | -0.0195

Frag |  3  |  Tertiary amine                            | -0.2880 | -0.8640

MolWt|  *  |  Molecular Weight Parameter                |         | -0.3772

Const|  *  |  Equation Constant                         |         |  3.8477

============+============================================+=========+=========

  RESULT   |   Biowin4 (Survey Model - Primary Biodeg)  |         |  2.5871

============+============================================+=========+=========

Result Classification:   5.00 -> hours     4.00 -> days    3.00 -> weeks

 (Primary & Ultimate)    2.00 -> months    1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM |       Biowin5 FRAGMENT DESCRIPTION         |  COEFF  |  VALUE  

------+-----+--------------------------------------------+---------+---------

Frag |  2  |  Aliphatic ether  [C-O-C]                  |  0.0015 |  0.0029

Frag |  3  |  Tertiary amine                            | -0.0848 | -0.2545

Frag |  5  |  Methyl  [-CH3]                            |  0.0004 |  0.0021

Frag |  8  |  -CH2-  [linear]                           |  0.0494 |  0.3953

MolWt|  *  |  Molecular Weight Parameter                |         | -0.7777

Const|  *  |  Equation Constant                         |         |  0.7121

============+============================================+=========+=========

  RESULT   |  Biowin5 (MITI Linear Biodeg Probability)  |         |  0.0803

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM |       Biowin6 FRAGMENT DESCRIPTION         |  COEFF  |  VALUE  

------+-----+--------------------------------------------+---------+---------

Frag |  2  |  Aliphatic ether  [C-O-C]                  | -0.1071 | -0.2143

Frag |  3  |  Tertiary amine                            | -0.8396 | -2.5189

Frag |  5  |  Methyl  [-CH3]                            |  0.0194 |  0.0971

Frag |  8  |  -CH2-  [linear]                           |  0.4295 |  3.4360

MolWt|  *  |  Molecular Weight Parameter                |         | -7.5466

============+============================================+=========+=========

  RESULT   |Biowin6 (MITI Non-Linear Biodeg Probability)|         |  0.0145

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM |       Biowin7 FRAGMENT DESCRIPTION         |  COEFF  |  VALUE  

------+-----+--------------------------------------------+---------+---------

Frag |  2  |  Aliphatic ether  [C-O-C]                  | -0.2573 | -0.5145

Frag |  3  |  Tertiary amine                            | -1.0749 | -3.2246

Frag |  5  |  Methyl  [-CH3]                            | -0.0796 | -0.3979

Frag |  8  |  -CH2-  [linear]                           |  0.0260 |  0.2079

Const|  *  |  Equation Constant                         |         |  0.8361

============+============================================+=========+=========

  RESULT   |   Biowin7 (Anaerobic Linear Biodeg Prob)   |         | -3.0929

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction:  If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable).  If this condition is not

satisfied, the prediction is NO (not readily biodegradable).  This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help).  Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The substance is predicted to be not readily biodegradable by EPI Suite BIOWIN v4.10.
Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018-03-05 to 2018-04-17
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Version / remarks:
1992
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
Version / remarks:
EC No. 440/2008
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 835.3200 (Zahn-Wellens / EMPA Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Information as provided by the Sponsor.
Identification: Jeffcat LE 30
CAS Number: 65286-55-7
Chemical Name: 2-(2-(dimethylamino)ethoxy)-N-(2-(2-(dimethylamino)ethoxy)ethyl)-N-methylethanamine
Physical state/Appearance: clear colorless liquid
Batch: DR74271215
Purity: 98.2%
Chemical Formula: C13H31N3O2
Label: Jeffcat LE 30 Lot no DR74271215 5,000L
Receipt Date: 25 January 2017
Expiry Date: 30 December 2018
Storage Conditions: room temperature, in the dark
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
A mixed population of activated sewage sludge micro-organisms was obtained on 19 March 2018 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.

Preparation of Inoculum
The sample of activated sewage sludge was maintained on continuous aeration upon receipt. A sample of the activated sewage sludge was washed twice by settlement and resuspension in mineral medium to remove any excessive amounts of Dissolved Organic Carbon (DOC) that may have been present. A sub-sample of the washed sewage sludge was then removed and the suspended solids concentration determined.
Duration of test (contact time):
28 d
Initial conc.:
167 mg/L
Based on:
test mat.
Initial conc.:
100 mg/L
Based on:
IC (inorganic carbon)
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Experimental Design and Study Conduct

Preliminary Investigational Work
During the test, samples are taken for DOC/TOC analysis and as part of the sample preparation the samples are either filtered or centrifuged to remove the sewage sludge solids. Thus the following work was conducted and samples analyzed for DOC/TOC using a Shimadzu TOC-VCPH TOC analyzer.
A nominal amount of test item (261 mg) was dissolved in mineral medium (1000 mL) to give a 261 mg/L stock solution. Two samples were taken for analysis; one untreated (TOC) and one filtered (DOC) through a 0.45 µm Gelman AcroCap filter (discarding the initial 5 mL to pre-condition the filter). A further nominal amount of test item (261 mg) was dissolved in mineral medium and inoculated at a concentration of 300 mg suspended solids (ss)/L prior to adjusting to a final volume of 1000 mL. Two samples were taken for DOC analysis; one after filtration through a Gelman 0.45 µm AcroCap filter (discarding the initial 5 mL to pre-condition the filter) and the other after centrifugation at 4000 g for 15 minutes. Control samples were prepared by inoculating mineral medium (1000 mL) at a suspended solids level of 300 mg ss/L and then filtering or centrifuging as per the test item samples.
For the inoculum, a mixed population of activated sewage sludge micro-organisms was obtained on 5 March 2018 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.

Test Item Preparation
The test item was dissolved directly in reverse osmosis purified deionized water prior to dispersal in inoculated mineral medium to give the required test concentration.
A nominal amount of test item (1670 mg) was dissolved in reverse osmosis purified deionized water and the volume adjusted to 1 liter to give a 1670 mg/L stock solution. An aliquot (200 mL) of the 1670 mg/L stock solution was dispersed in inoculated mineral medium and the volume adjusted to 2 liters to give a final test concentration of 167 mg/L, equivalent to 100 mg Carbon/L.
The volumetric flask containing the stock solution was inverted several times to ensure homogeneity of the solution.
The control group was maintained under identical conditions but not exposed to the test item.
A test concentration of 100 mg carbon/L was employed in the test following the recommendations of the Test Guidelines.

Reference Item Preparation
A reference item, diethylene glycol, was used to prepare the procedure control vessels. An initial stock solution of 2000 mg/L was prepared by dissolving the reference item directly in reverse osmosis purified deionized water. An aliquot (220 mL) of this stock solution was dispersed in inoculated mineral medium and the volume adjusted to 2 liters to give a final concentration of 220 mg/L, equivalent to 100 mg Carbon/L. The volumetric flask containing the reference item stock solution was inverted several times to ensure homogeneity of the solution.

Toxicity Control
A toxicity control, containing the test item and diethylene glycol was prepared in order to assess any toxic effect of the test item on the sewage sludge micro-organisms used in the test.
An aliquot (200 mL) of the 1670 mg/L test item stock solution was dispersed in inoculated mineral medium along with an aliquot (220 mL) of the 2000 mg/L reference item stock solution. The volume was adjusted to 2 liters to give a final concentration of 167 mg test item/L, plus 220 mg diethylene glycol/L, equivalent to a final concentration of 200 mg Carbon/L.

Preparation of Test System
The following test solutions were prepared and inoculated in 3 liter glass test vessels each containing 2 liters of solution:
a) An inoculum control, in duplicate, consisting of inoculated mineral medium.
b) The procedure control containing the reference item (diethylene glycol), in duplicate, in inoculated mineral medium to give a final concentration of 100 mg Carbon/L.
c) The test item, in duplicate, in inoculated mineral medium to give a final concentration of 100 mg Carbon/L.
d) The test item plus diethylene glycol, one replicate, in inoculated mineral medium to give a final concentration of 200 mg Carbon/L to act as a toxicity control.
Data from the inoculum control and procedure control vessels was shared with similar concurrent studies.

Each test vessel was inoculated with the prepared inoculum at a final concentration of 300 mg suspended solids/liter in order to give an inoculum to carbon ratio of 3:1.
The test vessels were covered with polypropylene lids to reduce evaporation and incubated at measured temperatures of between 20 and 25 ”C in darkness for 28 days. The room temperature was measured as being between 20 and 22 ºC during this time. The test vessels were constantly aerated with compressed air via glass tubes and stirred constantly by a magnetic stirrer.


Water Quality Criteria
Immediately prior to sampling the temperature and dissolved oxygen concentration (by means of a Hach HQ40d Flexi handheld meter) of each vessel were recorded. The pH of each vessel was also determined using a Hach HQ40d Flexi handheld meter and adjusted to pH 7 to 8, where necessary, with sodium hydroxide or sulphuric acid solutions.

Reference substance:
diethylene glycol
Remarks:
Physical state/Appearance: Clear colorless liquid Batch: 1665885 Purity: >99% Expiry Date: 09 May 2019 Storage Conditions: room temperature
Preliminary study:
The results obtained from the samples taken for organic carbon analysis from the preliminary investigational work indicated that the test item did not adsorb to filter matrices or to activated sewage sludge. Therefore, for the purpose of the study, the samples taken for DOC analysis were filtered to remove the suspended solids present without the loss of any test item
Key result
Parameter:
% degradation (DOC removal)
Value:
36
Sampling time:
28 d
Details on results:
From DOC analysis the test item attained 36% biodegradation after 28 days.
OECD Guideline No. 302B does not give any definitive pass levels for test items, however the “Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3 (2006)” suggests that a figure of more than 20% biodegradation may be regarded as evidence for inherent, primary biodegradability. A figure of more than 70% mineralization may be regarded as evidence for ultimate inherent biodegradation.
The test item can therefore be considered to have exhibited evidence of inherent, primary biodegradation under the experimental conditions employed in this study.
The procedure control, diethylene glycol attained 99% biodegradation after 14 days and 100% biodegradation after 28 days, thereby confirming the suitability of the inoculum and culture conditions.
The toxicity control attained 55% biodegradation after 14 days and 70% biodegradation after 28 days, therefore confirming that the test item was not toxic to the sewage treatment micro-organisms used in the study.
The pH values did not fall below 6.98 in any test vessel during the study and were readjusted to pH 7 to 8 where necessary on sampling days. Aerobic conditions in the test vessels were maintained throughout the study as dissolved oxygen concentrations remained at or above
7.24 mg O2/L in all culture vessels.

Table1            Dissolved Organic Carbon (DOC) Values on Each Sampling Occasion

 

Vessel

DOC (mg Carbon/L)

Day

0 Hours

3 Hours

1

7

14

21

27

28

Inoculum Control

R1

2.44

1.92

1.72

3.19

4.35

4.29

3.49

4.04

R2

1.65

1.77

1.70

3.92

4.10

3.75

4.44

3.90

Mean

2.05

1.85

1.71

3.56

4.23

4.02

3.97

3.97

Procedure Control

R1

104.40

105.70

95.66

5.19

5.15

4.78

4.49

4.38

R2

104.00

102.90

93.67

4.36

5.60

4.88

4.52

4.53

Mean

104.20

104.30

94.67

4.78

5.38

4.83

4.51

4.46

Test Item

R1

91.34

98.90

85.48

91.11

92.43

63.48

63.23

64.04

R2

93.89

94.79

86.60

94.87

95.22

65.37

64.48

65.81

Mean

92.62

96.85

86.04

92.99

93.83

64.43

63.86

64.93

Toxicity Control

200.60

198.10

179.30

93.75

93.25

63.69

61.39

63.68

R= Replicate

Table2            Percentage Biodegradation 

Day

% Biodegradation

Procedure Control

Test Item

Toxicity Control

1

9

11

10

7

99

6

54

14

99

6

55

21

99

36

70

27

99

37

71

28

100

36

70


Validity criteria fulfilled:
yes
Interpretation of results:
not inherently biodegradable
Conclusions:
The test item attained 36% biodegradation after 28 days.
OECD Guideline No. 302B does not give any definitive pass levels for test items, however the “Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3 (2006)” suggests that a figure of more than 20% biodegradation may be regarded as evidence for inherent, primary biodegradability. A figure of more than 70% mineralization may be regarded as evidence for ultimate inherent biodegradation.
The test item can therefore be considered to have exhibited evidence of inherent, primary biodegradation under the experimental conditions employed in this study.

Executive summary:

The study was performed to assess the inherent biodegradability of the test item in an aerobic aqueous media. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 302B, “Inherent Biodegradability; Modified Zahn-Wellens/EMPA Test”, Method C.9 of Commission Regulation (EC) No. 440/2008, and the US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3200.

 Methods

The test item was exposed to activated sewage sludge micro-organisms at a concentration of 100 mg carbon/L with mineral medium in the dark at measured temperatures of between 20 and 25ºC for 28 days. The biodegradation of the test item was assessed by the determination of dissolved organic carbon removal. Control cultures with inoculum and the reference item, diethylene glycol, together with a toxicity control were used for validation purposes.

 Results

The test item attained 36% biodegradation after 28 days.

OECD Guideline No. 302B does not give any definitive pass levels for test items, however the “Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3 (2006)” suggests that a figure of more than 20% biodegradation may be regarded as evidence for inherent, primary biodegradability. A figure of more than 70% mineralization may be regarded as evidence for ultimate inherent biodegradation.

The test item can therefore be considered to have exhibited evidence of inherent, primary biodegradation under the experimental conditions employed in this study.

Description of key information

The ready biodegradation potential of the substance was assessed with a QSAR analysis (using the BIOWIN model). The results indicated that the substance is considered to be not readily biodegradable.


 


The inherent biodegradation of the substance was determined in a study carried out according to OECD 302B, EU Method C.9 and EPAOPPTS 835.3110. Based on DOC analysis the test item attained 6% biodegradation after 14 days and 36% biodegradation after 28 days exposure time. The test item is considered to have exhibited no evidence of ultimate biodegradation under the experimental conditions employed in this study. Based on these results, the substance is considered to be not inherently biodegradable. 


 

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable
Type of water:
freshwater

Additional information