Chrome antimony titanium buff rutile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1999-11-03 to 1999-11-05

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

no analysis of test concentrations; the dissolved fraction of the test material used for the preparation of test solutions was established by stirring the test material for only 20 hours

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

1996

Test guideline 4

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Version / remarks:

1989

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Chrome antimony titanium buff rutile

EC Number:

269-052-1

EC Name:

Chrome antimony titanium buff rutile

Cas Number:

68186-90-3

Molecular formula:

(Ti, Sb, Cr) O2

IUPAC Name:

manganese(4+) trititanium(4+) pentaantimony(3+) chromium(3+) nickel(2+) octadecaoxidandiide

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
The insoluble test substance (water solubility < 1 mg/L) was stirred in the M4 medium (see dilution water) for ca. 20 h at ca. 20 °C. Undissolved test substance was removed by filtration with a membrane filter (pore width 0.2 µm). The nominal concentration of the filtrate was 100 mg/L. Further dilution of this filtrate with M4 medium led to the nominal concentrations: 12.5, 25, and 50 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquee, France, in 1978. From this date on, this clone was cultured and bred continuously in the Laboratory of Ecology of BASF AG in Ludwigshafen
- Age at study initiation: 2- 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L

Test temperature:

20.4 - 20.5 °C

pH:

8.1 - 8.5

Dissolved oxygen:

8.2 - 8.5 mg/L

Nominal and measured concentrations:

Nominal concentrations: control, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass with flat bottom (nominal volume 20 mL)
- fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/mL


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
Synthetic fresh water was used for culture and test purposes. This water was prepared on the basis of an ultrapure, demineralized water with a conductivity < 0 .05 µS/cm. For the composition of this M4 medium see ISO10706.
Properties of this medium:
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L
Molar ratio Ca:Mg about 4 : 1
pH value: 7.5 - 8.5
Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: day:night rhythm = 16 : 8 h
- Light intensity: type warm white (e.g. OSRAM L58 W31), about 2 - 7 µE/(m2*s) at a wave length of 400 to 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Check of swimming ability: Visually after 0, 24 and 48 h
Measurement of temperature: Continuously during the whole test period in a separate vessel close to the test vessels.
Measurement of pH and oxygen: After 0 h in an additional replicate without daphnids and after 48 h in replicate 1 of each tested concentration.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Details on results:

Effect data (Immobilisation): No immobilisation at any dose group or control. No other effects were observed.

Results with reference substance (positive control):

Positive control performed with potassium dichromate: EC50, 24 h was 1.01 mg/L.

Reported statistics and error estimates:

Results allowed no statistical evaluation of the data.

Applicant's summary and conclusion

Conclusions:

Dissolved fractions of chrome antimony titanium buff rutile are not acutely toxic to Daphnia magna in a test according to OECD TG 202 with dissolved fractions prepared at nominal concentrations far exceeding its water solubility. The corresponding EC50 for immobilisation is estimated with > 100 mg/L (nominal). However, since the dissolved fraction for the preparation of test solutions was prepared by stirring the test substance for only 20 hours and no chemical analysis was performed, the reliability of the study is rated RL3.