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EC number: 239-701-3
CAS number: 15625-89-5
study was performed to investigate the potential of Trimethylolpropane
triacrylate (TMPTA) to induce micronuclei in bone marrow cells of mice.
The study was performed according to guidelines (OECD 474, Commission
Directive No. B12, OPPTS 870.5395 and Japanese guidelines) and in
compliance with the Principles of Good Laboratory Practice.
preliminary toxicity test was performed to define the highest possible
dose to be used for the cytogenetic study. In the main study, four
groups of five male and five female Swiss Ico: OF1 (IOPS Caw) mice
received a single oral administration of the test material at the
following doses: 437.5, 875 and 1750 mg/kg bw (for males) or 500, 1000
and 2000 mg/kg bw (for females). Two groups of five males and five
females received the vehicle (corn oil) acted as the control groups. One
group of five males and five females received Cyclophosphamide (positive
control) once by the oral route at a dose of 50 mg/kg bw. The animals
were killed 24 (control, low, intermediate and high dose groups) or 48 h
(control and high dose groups) after treatment. The animals of the
positive control group were killed 24 h after treatment. Bone marrow
smears were then prepared. For each animal, the number of the
micronucleated polychromatic erythrocytes (MPE) was counted in 2000
polychromatic erythrocytes. The polychromatic (PE) and normochromatic
(NE) erythrocyte ratio was established by scoring a total of 1000
erythrocytes (PE + NE).
males, no clinical signs and no mortality attributed to the treatment
were observed in controls and 437.5 mg/kg bw group. At 875 mg/kg bw,
piloerection was noted and at 1750 mg/kg bw, two males were found dead
24 h following the treatment and piloerection was noted at the same time
in the surviving males. In females, no clinical signs and no mortality
attributed to the treatment were observed in animals given 0, 500, 1000
or 2000 mg/kg bw.
values of MPE as well as the PE/NE ratios in the treated group were
equivalent to those of the control group, for both harvest times. All
frequencies of micronucleated cells (MPE) obtained in treated animals
were clearly within or consistent with the historical vehicle control
range. Cyclophosphamide induced a significant increase in the frequency
of MPE, demonstrating the sensitivity of the test system under the
experimental conditions of this study.
conclusion trimethylolpropane triacrylate (TMPTA) was considered to be
non-mutagenic in the micronucleus test.
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