2-ethyl-2-[[(1-oxoallyl)oxy]methyl]-1,3-propanediyl diacrylate; 2,2-bis(acryloyloxymethyl)butyl acrylate; trimethylolpropane triacrylate

Short-term toxicity to fish

Description of key information

In the key study, acute toxicity to Danio rerio was determined at 0.87 mg/L.

The acute toxicity of the test substance to Danio rerio exposed under semi-static conditions for 96h was assessed according to the OECD 203 Guideline. Fish were exposed to a series of test solutions renewed every day throughout the test period. Test item concentrations were determined at each observation time on fresh and old solutions: as chemical analysis of test samples taken on fresh and old solutions indicated that measured concentrations of the test item did not remain within 80-120% of the nominal concentrations and were not maintained over a 24h test period (i.e. deviation >20%), the exposure concentrations were based on the geometric mean of measured concentrations and determined as follows:

96h-NOEC       0.89 mg/L

96h-LOEC       1.71 mg/L

96h-LC10       0.75 mg/L

96h-LC50       0.87 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.87 mg/L

Additional information

Two test records for fish toxicity are available. The first is reported as the key study and is described above. The second study was not performed according to GLP and is used as supplementary information.

The substance was tested for acute fish toxicity in Leuciscus idus according to DIN 38412/15. After 96 h exposure to nominal concentrations of 0.1, 0.215, 0.464, 1 and 2.15 mg/l an LC 50 was determined to be between 1 mg /l (no mortality) and 2.15 mg/l (100% mortality). The NOEC was 1 mg/l.

Since no mortality occurred between 0.1 and 1 mg/l the geometric mean between the highest concentration without effect and the lowest concentration with 100% effect were considered to determine the LC50 to be 1.47 mg/l.