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EC number: 270-790-1
CAS number: 68478-10-4
A complex combination of hydrocarbons obtained by the distillation of debenzenized light steam-cracked naphtha. It consists predominantly of cyclic olefinic and aromatic hydrocarbons having carbon numbers predominantly in the range of C8 through C16 and boiling in the range of approximately 130°C to 300°C (226°F to 572°F).
key studies are considered to be a bacterial mutation assay (NOTOX,
2000), a mammalian cell cytogenetic assay (JETOC, 1998b), and a
mammalian gene mutation assay (Harlan, 2014). These
are three recognised core assay types for investigating mutation in
(resin grade containing 75% dicyclopentadiene) was tested in a
pre-incubation modification of a standard Ames test (NOTOX
TA1537, TA98 and TA100) and E. coli (WP2uvrA) were treated with
dicyclopentadiene both with and without auxiliary metabolic activation
range of doses was used up to 666 μg/plate where toxicity allowed, and
with S9 levels of 5% and/or 10%. Dicyclopentadiene was negative in this
was tested for cytogenetic activity in CHL
cells both in
the presence and absence of S9 (JETOC, 1998b). A
range of doses up to approximately 0.1 mg/ml (approximately 10 mM) was
used, and both a continuous and short term exposure was employed in the
absence of S9. Dicyclopentadiene
was negative in this assay at concentrations causing up to 50%
inhibition of cell growth, in both the absence and presence of S9. A
small increase in aberrations was observed at a high concentration on
continuous exposure in the absence of S9. This
small increase is not considered to be significant, and
dicyclopentadiene was reported as negative.
Dicyclopentadiene was tested for gene
mutation potential in L5178Y mouse lymphoma cells, both in the absence
and presence of S9 (Harlan, 2014). An initial toxicity test indicated
that concentrations would be limited by cyctoxicity to ca. 40-60μg/mL.
Dicyclopentadiene did not induce a statistically significant,
dose-related increase in mutant frequency, and was considered to be
negative in this test.
are additional reports of negative results for dicyclopentadiene in an in
vitro mammalian cell micronucleus assay (JETOC, 1998b), in the Ames
test (+/- S9) in Salmonella strains TA1535, TA1537, TA1538, TA98 and
TA100 (Litton Bionetics 1980), in Salmonella strains TA1535, TA1537,
TA98 and TA100 (Litton
Bionetics 1980 )
and in S cerevisiae D4 (+/- S9) (Litton
and S cerevisiae D3 (Litton
data from these assays all support the above conclusion that
dicyclopentadiene is not mutagenic in vitro.
are no available in vivo studies on dicyclopentadiene, however there is
an important supporting bone marrow micronucleus study in the mouse
available on dicyclopentadiene / codimer concentrate, containing 29.175%
dicyclopentadiene (DuPont, 2004). This
is a recognised core assay type for investigating mutation in vivo.
and female mice were given two doses of dicyclopentadiene / codimer
concentrate by oral gavage, 24h apart, and bone marrow sampled 24h after
the last dose. The
dose levels used were 0, 437, 875, or 1750 mg/kg, and the highest dose
induced clinical signs in both sexes and evidence of bone marrow
toxicity (decreased PCE/NCE ratio) in females. A
negative result was obtained in this assay.
is no information indicating any adverse effects of dicyclopentadiene.
Short description of key
Dicyclopentadiene has been evaluated for mutagenicity both in vitro
and in vivo using recognised core assay studies and has shown negative
results. It is concluded from the available data that dicyclopentadiene
has no significant genotoxicity.
Endpoint Conclusion: No adverse effect observed (negative)
It is concluded that the available data
indicate that dicyclopentadiene has no significant genotoxicity and
therefore does not warrant classification for mutagenicity under CLP.
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