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EC number: 278-770-4
CAS number: 77804-81-0
effects, evident as a reduction in the number of revertants, were observed
at the following concentrations (µg/plate).
without S9 mix
with S9 mix
1000 - 5000
/ = no
toxic effects observed
The test item
precipitated in the overlay agar at the following concentrations
particles had no influence on the data recording.
Mutagenic activity of the test item was investigated in Salmonella
typhimurium strains TA 1535, TA 1537, TA98 and TA100 as well as
Escherichia coli strain WP2 uvrA with (induced rat liver S9 mix) and
without metabolic activation at concentrations of 3, 10, 33, 100, 333,
1000, 2500 and 5000 µg/plate using the plate incorporation assay. Due to
the test items characteristic as an azo-dye the test was also conducted
using the Prival modification, i.e. testing the above mentioned
bacterial strains in the preincubation assay without and with uninduced
hamster liver S9 mix for metabolic activation. This test was performed
using the concentrations 10, 33, 100, 333, 1000, 2500 and 5000 µg/plate.
The test item did not reveal any mutagenic activity under the conditions
tested. The appropriate reference mutagenes showed distinct positive
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