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Diss Factsheets
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EC number: 202-297-4 | CAS number: 94-04-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicological Summary
- Administrative data
- Workers - Hazard via inhalation route
- Workers - Hazard via dermal route
- Workers - Hazard for the eyes
- Additional information - workers
- General Population - Hazard via inhalation route
- General Population - Hazard via dermal route
- General Population - Hazard via oral route
- General Population - Hazard for the eyes
- Additional information - General Population
Administrative data
Workers - Hazard via inhalation route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- DNEL (Derived No Effect Level)
- Value:
- 9.8 mg/m³
- Most sensitive endpoint:
- repeated dose toxicity
DNEL related information
- Overall assessment factor (AF):
- 18
- Modified dose descriptor starting point:
- NOAEC
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
Workers - Hazard via dermal route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- DNEL (Derived No Effect Level)
- Value:
- 23.59 mg/kg bw/day
- Most sensitive endpoint:
- repeated dose toxicity
DNEL related information
- Overall assessment factor (AF):
- 43
- Modified dose descriptor starting point:
- NOAEL
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- DNEL (Derived No Effect Level)
- Value:
- 198 µg/cm²
- Most sensitive endpoint:
- sensitisation (skin)
DNEL related information
- DNEL derivation method:
- ECHA REACH Guidance
- Overall assessment factor (AF):
- 63
- Dose descriptor:
- other: LOAEL
- AF for dose response relationship:
- 3
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health, Section r.8.4.3.1. page 35 and Appendix R. 8-10 page 128.
- AF for differences in duration of exposure:
- 1
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health Section R.8.4.3.1. page 35. .
- AF for interspecies differences (allometric scaling):
- 7
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health Section R.8.4.3. Table R. 8-3, page 30 and Table R. 8-4, page 32.
- AF for other interspecies differences:
- 1
- Justification:
- Allometric scaling was used.
- AF for intraspecies differences:
- 3
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health Appendix R. 8-3, Table R. 8-19, page 76.
- AF for the quality of the whole database:
- 1
- Justification:
- Generally, all studies are of high quality Klimisch 1 with GLP compliance.
- Justification:
- NA
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
Workers - Hazard for the eyes
Local effects
- Hazard assessment conclusion:
- no hazard identified
Additional information - workers
The NOAEL in an OECD 408 90-day repeat dose toxicity study in rats was 500 mg/kg/day.
The REACH Technical Guidance Document R.8 contains default assessment factors which should be applied to a modified dose descriptor in order to obtain a DNEL. These factors are multiplicative and can lead to a human chronic NAEL that is 100 or 200 fold lower than the equivalent rat subchronic NOAEL. However other guidance is available from ECETOC (2003), which supports smaller assessment factors to account for inter- and intra- species differences; leading to a human chronic NAEL that is only 24 to 40 -fold lower than an equivalent rat sub\chronic NOAEL. The ECETOC technical report includes scientific justification for the magnitude of these assessment factors, including:
· Although “residual” interspecies variability may remain following allometric scaling, this is largely accounted for in the default assessment factor proposed for intraspecies variability.
· Following analysis of the inherent variability in human toxicokinetic and toxicodynamic parameters, a difference of 3 (close to the 90th percentile) was considered appropriate to account for variability present in worker groups while a value of 5 (equivalent to the 95th percentile) was appropriate for the general population.
Section R.8.4.3.3 of the REACH Technical Guidance Document recognizes that the overall assessment factor applied to an experimental NOAEL when developing a DNEL is multiplicative in nature, and that “Care should be taken to avoid double counting several aspects when multiplying the individual factors.” Based on the information presented in ECETOC (2003) and summarized above, use of the standard defaults for inter- and intra- species variability contained in REACH Technical Guidance Document appears to result in “double counting”, and if used inappropriately, would lead to a large, conservative overall assessment factor. In order to retain the scientific credibility in its DNEL setting process, we will adopt the assessment factors proposed by ECETOC (2003) when developing DNELs.
Critical NOAEL used = rabbit oral NOAEL 100 mg/kg/day (developmental toxicity study (maternal toxicity) – systemic)
Absorption rates: 10% dermal, 100% oral, and 100% inhalation regardless of species
Dermal - Systemic
Dose descriptor = NOAEL of 100 mg/kg/day; from a well described oral developmental toxicity study.
Modification of dose descriptor
= oral NOAEL * (rat oral absorption rate / human dermal absorption rate)
= 100 * (100% / 10%)
dermal NOAEL = 1000 mg/kg/day
Adjustment factors (ECETOC Technical Report #86):
Criteria | Adjustment Factor |
Allometric scaling - rabbit | 2.4 |
Intraspecies - workers | 3 |
Subacute study to Chronic | 6 |
Overall Adjustment Factor | 43.2 |
DNEL calculation
DNEL dermal = dermal NOAEL / (adjustment factors)
DNEL dermal = 1000 mg/kg/day / (2.4 * 3 * 6)
DNEL dermal = 23.15 mg/kg/day
Inhalation
Dose descriptor = NOAEL of 100 mg/kg/day; from a well described oral developmental toxicity study
Route-to-route extrapolation (calculation B.3 in ECHA Guidance R.8)
inhalatory NOAEC = oral LOAEL * (1/ sRV rat 8h) * (ABS oral / ABS inh) * (sRV human / wRV)
inhalatory NOAEC= 100 * (1/0.38) * (100/100) * (6.7/10)
inhalatory NOAEC = 176.32 mg/m3
Adjustment factors (ECETOC Technical Report #86):
Criteria | Adjustment Factor |
Allometric scaling - rabbit | not applied |
Intraspecies - workers | 3 |
Subacute study to Chronic | 6 |
Overall Adjustment Factor | 18 |
DNEL calculation
DNEL inhalation = inhalation NOAEC / (adjustment factors)
DNEL inhalation = 176.32 mg/m3 / (18)
DNEL inhalation = 9.80 mg/m3
There were no local effects observed in repeated-dose rat studies upon which long-term exposure-local effects DNELs could be established.
A dermal local effects DNEL is based up on the outcome of an O.E.C.D. test guideline Mouse LLNA with an EC3 value of approximately 50 % v/v. Appropriate E.C.H.A. guidance recommended Assessment Factors were employeed to derive the DNEL value.
General Population - Hazard via inhalation route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- DNEL (Derived No Effect Level)
- Value:
- 2.9 mg/m³
- Most sensitive endpoint:
- repeated dose toxicity
DNEL related information
- Overall assessment factor (AF):
- 30
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- no hazard identified
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
General Population - Hazard via dermal route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- DNEL (Derived No Effect Level)
- Value:
- 13.89 mg/kg bw/day
- Most sensitive endpoint:
- repeated dose toxicity
DNEL related information
- Overall assessment factor (AF):
- 72
- Modified dose descriptor starting point:
- NOAEL
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
Local effects
Long term exposure
- Hazard assessment conclusion:
- DNEL (Derived No Effect Level)
- Value:
- 119 µg/cm²
- Most sensitive endpoint:
- sensitisation (skin)
DNEL related information
- DNEL derivation method:
- ECHA REACH Guidance
- Overall assessment factor (AF):
- 105
- Dose descriptor:
- other: LOAEL
- AF for dose response relationship:
- 3
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health Section R.8.4.3.1, page 36 and Appendix R. 8-10, page 128.
- AF for differences in duration of exposure:
- 1
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health Section R.8.4.3.1. page 35.
- AF for interspecies differences (allometric scaling):
- 7
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health, Section R. 8.4.3. Table R 8-3, page 30 and Table R. 8-4, page 32.
- AF for other interspecies differences:
- 1
- Justification:
- Allometric Scaling was used.
- AF for intraspecies differences:
- 5
- Justification:
- According to Guidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health. Appendix 8.3. Table R. 8-19, page 76.
- AF for the quality of the whole database:
- 1
- Justification:
- Generally, all studies are of high quality, Klimisch1 with GLP compliance.
- Justification:
- NA
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
General Population - Hazard via oral route
Systemic effects
Long term exposure
- Hazard assessment conclusion:
- DNEL (Derived No Effect Level)
- Value:
- 1.39 mg/kg bw/day
- Most sensitive endpoint:
- repeated dose toxicity
DNEL related information
- Overall assessment factor (AF):
- 72
- Modified dose descriptor starting point:
- NOAEL
Acute/short term exposure
- Hazard assessment conclusion:
- no hazard identified
DNEL related information
General Population - Hazard for the eyes
Local effects
- Hazard assessment conclusion:
- no hazard identified
Additional information - General Population
The NOAEL in an OECD 408 90-day repeat dose toxicity study in rats was 500 mg/kg/day.
The REACH Technical Guidance Document R.8 contains default assessment factors which should be applied to a modified dose descriptor in order to obtain a DNEL. These factors are multiplicative and can lead to a human chronic NAEL that is 100 or 200 fold lower than the equivalent rat subchronic NOAEL. However other guidance is available from ECETOC (2003), which supports smaller assessment factors to account for inter- and intra- species differences; leading to a human chronic NAEL that is only 24 to 40 -fold lower than an equivalent rat sub\chronic NOAEL. The ECETOC technical report includes scientific justification for the magnitude of these assessment factors, including:
· Although “residual” interspecies variability may remain following allometric scaling, this is largely accounted for in the default assessment factor proposed for intraspecies variability.
· Following analysis of the inherent variability in human toxicokinetic and toxicodynamic parameters, a difference of 3 (close to the 90th percentile) was considered appropriate to account for variability present in worker groups while a value of 5 (equivalent to the 95th percentile) was appropriate for the general population.
Section R.8.4.3.3 of the REACH Technical Guidance Document recognizes that the overall assessment factor applied to an experimental NOAEL when developing a DNEL is multiplicative in nature, and that “Care should be taken to avoid double counting several aspects when multiplying the individual factors.” Based on the information presented in ECETOC (2003) and summarized above, use of the standard defaults for inter- and intra- species variability contained in REACH Technical Guidance Document appears to result in “double counting”, and if used inappropriately, would lead to a large, conservative overall assessment factor. In order to retain the scientific credibility in its DNEL setting process, we will adopt the assessment factors proposed by ECETOC (2003) when developing DNELs.
Critical NOAEL used = rabbit oral NOAEL 100 mg/kg/day (developmental toxicity study (maternal toxicity) – systemic)
Absorption rates: 10% dermal, 100% oral, and 100% inhalation regardless of species
Dermal - Systemic
Dose descriptor = NOAEL of 100 mg/kg/day; from a well described oral developmental toxicity study.
Modification of dose descriptor
= dermal NOAEL * (rat oral absorption rate / human dermal absorption rate)
= 100 * (100% / 10%)
dermal NOAEL = 1000 mg/kg/day
Adjustment factors (ECETOC Technical Report #86):
Criteria | Adjustment Factor |
Allometric scaling - rabbit | 2.4 |
Intraspecies – general population | 5 |
Subacute study to Chronic | 6 |
Overall Adjustment Factor | 72 |
DNEL calculation
DNEL dermal = dermal NOAEL / (adjustment factors)
DNEL dermal = 1000 mg/kg/day / (2.4 * 5 * 6)
DNEL dermal = 13.89 mg/kg/day
Inhalation
Dose descriptor = NOAEL of 100 mg/kg/day; from a well described development toxicity study
Route-to-route extrapolation (calculation B.3 in ECHA Guidance R.8)
inhalatory NOAEC = oral NOAEL * (1/ sRV rat 24h) * (ABS oral / ABS inh)
inhalatory NOAEC= 100 * (1/1.15) * (100/100)
inhalatory NOAEC = 86.96 mg/m3
Adjustment factors (ECETOC Technical Report #86):
Criteria | Adjustment Factor |
Allometric scaling - rabbit | not applied |
Intraspecies – General Population | 5 |
Subacute study to Chronic | 6 |
Overall Adjustment Factor | 30 |
DNEL calculation
DNEL inhalation = inhalation NOAEC / (adjustment factors)
DNEL inhalation = 86.96 mg/m3 / (30)
DNEL inhalation = 2.90 mg/m3
Oral
Dose descriptor = NOAEL of 100 mg/kg/day; from a well described oral development toxicity study
Modification of dose descriptor: Not required
Adjustment factors (ECETOC Technical Report #86):
Criteria | Adjustment Factor |
Allometric scaling - rabbit | 2.4 |
Intraspecies – General Population | 5 |
Subacute study to Chronic | 6 |
Overall Adjustment Factor | 72 |
DNEL calculation
DNEL oral = oral NOAEL / (adjustment factors)
DNEL oral = 100 mg/kg/day / (72)
DNEL oral = 1.39 mg/kg/day
There were no local effects observed in repeated-dose rat studies upon which long-term exposure-local effects DNELs could be established.
A dermal local effects DNEL for the General Population is based up on the outcome of an O.E.C.D. test guideline Mouse LLNA with an EC3 value of approximately 50 % v/v. The dermal DNEL was derived following the E.C.H.A. guidance document, "
According toGuidance on information requirements and chemical safety assessment Chapter R.8. Characterisation of dose [concentration]-response for human health."
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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