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Ecotoxicological information

Long-term toxicity to fish

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Reference
Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to
Guideline:
other: Refer below principle
Principles of method if other than guideline:
Long term toxicity to fish studies were conduted for 35 days in Pimephales promelas to evaluate the toxicity of test chemical.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Pimephales notatus
Test type:
not specified
Water media type:
not specified
Total exposure duration:
35 d
Reference substance (positive control):
not specified
Key result
Duration:
35 d
Dose descriptor:
NOEC
Effect conc.:
0.005 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: Hatchability of eggs, growth, and survival
Remarks on result:
other: Other details not known
Validity criteria fulfilled:
not specified
Conclusions:
The No observed effect concentration (NOEC) value of test chemical in 35 day study in Pimephales promelas was observed to be 0.005 mg/L on basis of effect on Hatchability of eggs, growth, and survival.
Executive summary:

35 days long term fish toxicity study was conducted to test the effects of test chemical on Pimephales promelas. The No observed effect concentration (NOEC) value of test chemical in 35 day study in Pimephales promelas was observed to be 0.005 mg/L on basis of effect on Hatchability of eggs, growth, and survival.

Description of key information

35 days long term fish toxicity study was conducted to test the effects of test chemical on Pimephales promelas. The No observed effect concentration (NOEC) value of test chemical in 35 day study in Pimephales promelas was observed to be 0.005 mg/L on basis of effect on Hatchability of eggs, growth, and survival.

First study was supported by the second study. Long term fish toxicity study was conducted to test the effects of test chemical on danio rerio larvae in 9 days. At least three replicates of 15 embryos were placed in 50 ml beaker for five concentrations. The solutions were replaced every 48 h (i.e. a semi-static system) and the concentration of the selected antimicrobials was monitored using the HPLC as presented above. The DO and pH were also monitored at the replacement of the solutions. The percentage hatching and the survival rate of the larvae were evaluated until the egg yolk had disappeared. The No observed effect concentration (NOEC) value of test chemical in 9 day study in Danio rerio larvae was observed to be 0.024 mg/L on basis of effect on hatching and the survival of larvae.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
0.024 mg/L

Additional information

Various long term studies available for the test chemical were reviewed to determine the toxic nature of test chemical on the mortality of fish. The studies are as mentioned below:

35 days long term fish toxicity study was conducted to test the effects of test chemical on Pimephales promelas. The No observed effect concentration (NOEC) value of test chemical in 35 day study in Pimephales promelas was observed to be 0.005 mg/L on basis of effect on Hatchability of eggs, growth, and survival.

First study was supported by the second study. Long term fish toxicity study was conducted to test the effects of test chemical on danio rerio larvae in 9 days. At least three replicates of 15 embryos were placed in 50 ml beaker for five concentrations. The solutions were replaced every 48 h (i.e. a semi-static system) and the concentration of the selected antimicrobials was monitored using the HPLC as presented above. The DO and pH were also monitored at the replacement of the solutions. The percentage hatching and the survival rate of the larvae were evaluated until the egg yolk had disappeared. The No observed effect concentration (NOEC) value of test chemical in 9 day study in Danio rerio larvae was observed to be 0.024 mg/L on basis of effect on hatching and the survival of larvae.

 

Aim of this study was to evaluate the effect of test chemical on mortality of Pimephales promelas. Test conducted under the flow-through system. The Low  observed effect level (LOEL) value of test chemical in 30 day study in Pimephales promelas on basis of effect on mortality was determine to be 0.01 mg/L.

 

Similar 35 days long term fish toxicity study was conducted to test the effects of test chemical on pimpheles promelas. No treatment related effects were observed at the used concentrations. The No observed effect concentration (NOEC) of test chemical in 35 day study in Pimephales promelas on basis of effect on egg hatchability or growth of the fry was observed to be 0.005 mg/L.

 

Thus based on the above results, chemical consider to be toxic and classified in aquatic chronic category 2 as per the CLP classification criteria.