Registration Dossier

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to
Guideline:
EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)
Principles of method if other than guideline:
Study was conducted to determine the effect of test chemical on Eisenia fetida
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
No Data
Vehicle:
yes
Details on preparation and application of test substrate:
No Data
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name:Eisenia fetida
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
NO data
Test temperature:
22 Deg C
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
40 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Other details not known
Details on results:
No data
Results with reference substance (positive control):
No data
Reported statistics and error estimates:
No data
Validity criteria fulfilled:
not specified
Conclusions:
The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.
Executive summary:

Study was conducted to determine the effect of test chemical on Eisenia fetida. The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.

Description of key information

The first study was conducted to determine the effect of test chemical on Eisenia fetida. The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
40 mg/kg soil dw

Additional information

Toxicity to soil microorganisms except arthropods:

Summarized result for the toxicity of test chemical were studied on the soil microorganisms. The studies are as mention below:

 

The first study was conducted to determine the effect of test chemical on Eisenia fetida. The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.

 

First study was supported by the second study from peer reviewed journal. The effects of test chemical were studied in the freshwater mudsnail Potamopyrgus antipodarum over 4 weeks were determined. Aquatic vegetation was collected in D-nets, and snails were removed and kept in river water in 1- gallon buckets for transfer to the laboratory. Within 2 h, snails were transferred into 10-gallon aquaria filled with artificial freshwater (Milli-Q water plus added salts at a rate of 5 g CaCO3, 5 g sea salt, and 0.5 g NaHCO3 per 10 gallons of water). After 3 to 5 d of acclimation to laboratory conditions, 60 individuals with shell lengths greater than 3.0 mm were transferred to each individual 1-L jar filled with 800 ml artificial freshwater. The jars were aerated through glass pipettes to keep dissolved oxygen near saturation. After four weeks, environmentally relevant test chemical concentrations of 1.6 to 10.5 mg/L resulted in statistically significant increases in the number of unshelled embryos, whereas 0.2, 1.6, and 10.5 mg/L exposures significantly increased numbers of shelled embryos. The lowest observed effect concentration (LOEC) was 0.2 mg/L, the no observed effect concentration (NOEC) was 0.05 mg/L; the 10% effective concentration (EC10) and the median effective concentration (EC50) for unshelled effects were 0.5 mg/L and 2.5 mg/L, respectively.