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EC number: 202-924-1
CAS number: 101-20-2
Study was conducted to determine the
effect of test chemical on Eisenia fetida. The Lethal effect
concentration (LC50) value of test chemical to Eisenia fetida in a 48
hrs study was determine to be 40 mg/kg.
The first study was conducted to
determine the effect of test chemical on Eisenia fetida. The Lethal
effect concentration (LC50) value of test chemical to Eisenia fetida in
a 48 hrs study was determine to be 40 mg/kg.
Toxicity to soil microorganisms
Summarized result for the toxicity of
test chemical were studied on the soil microorganisms. The studies are
as mention below:
First study was supported by the
second study from peer reviewed journal. The effects of test chemical
were studied in the freshwater mudsnail Potamopyrgus antipodarum over 4
weeks were determined. Aquatic vegetation was collected in D-nets, and
snails were removed and kept in river water in 1- gallon buckets for
transfer to the laboratory. Within 2 h, snails were transferred into
10-gallon aquaria filled with artificial freshwater (Milli-Q water plus
added salts at a rate of 5 g CaCO3, 5 g sea salt, and 0.5 g NaHCO3 per
10 gallons of water). After 3 to 5 d of acclimation to laboratory
conditions, 60 individuals with shell lengths greater than 3.0 mm were
transferred to each individual 1-L jar filled with 800 ml artificial
freshwater. The jars were aerated through glass pipettes to keep
dissolved oxygen near saturation. After four weeks, environmentally
relevant test chemical concentrations of 1.6 to 10.5 mg/L resulted in
statistically significant increases in the number of unshelled embryos,
whereas 0.2, 1.6, and 10.5 mg/L exposures significantly increased
numbers of shelled embryos. The lowest observed effect concentration
(LOEC) was 0.2 mg/L, the no observed effect concentration (NOEC) was
0.05 mg/L; the 10% effective concentration (EC10) and the median
effective concentration (EC50) for unshelled effects were 0.5 mg/L and
2.5 mg/L, respectively.
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