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EC number: 202-924-1
CAS number: 101-20-2
Acute toxicity of test chemical on
fish Oryzias latipes were studied under the static system. Static system
were used for the study. Fish acute toxicity was tested using Japanese
medaka (Oryzias latipes) purchased from the National Institute for
Environmental Studies and acclimated in the laboratory of the University
of Tokushima at least for 3 months. The acute tests were conducted in
conformity to the OECD guideline for testing chemicals no. 203.
Duplicates of five juvenile fish were prepared in a 100 ml beaker for at
least five concentrations and blanks (and solvent blanks for TCC). The
test was conducted at 25 °C with a photoperiod of 16 h light and 8 h
dark. The solution was replaced at 48 h and the concentrations of the
tested antimicrobials were analyzed using the HPLC. The DO and pH of the
solution were checked at 0, 48, and 96 h. The number of dead individuals
was counted at 96 h and the median lethal concentration (LC50) was
determined on the basis of geometrical mean of the measured
concentrations of the tested antimicrobials using probit or logit
conversion with Ecotox-Statics version 2.6d. The Lethal effect
concentration (LC50) value of test chemical in fish in a 96 hr study on
the basis of mortality effect was observed to be 0.085 mg/L. Thus based
on the LC50 value, chemical consider to be toxic and classified as
aquatic acute 1 category as per the CLP classification criteria.
Based on the short term toxicity study
available for the test chemical, studies has were reviewed to determine
the toxic nature of test chemical on the mortality of fish. The studies
are as mentioned below:
First study was supported by the
second and third study from peer reviewed journal. The exposed adult
fathead minnows (Pimephales promelas) for 48 hours to test chemical were
studied. Chemicals were added to the conditioned water after water
exchanges (100% static renewal). The second day of exposure, fish were
removed from the tanks and placed in beakers that were filled with the
tank’s water and with air stones. Then, tanks were emptied, and filled
with new conditioned water. Chemicals were added to the conditioned
water and fish were placed again in the tanks. This was done in order to
maintain constant chemical concentrations in the media during the 48
hours of exposure. Three water samples were collected from each tank in
pre-rinsed 40 mL amber glass vials with Teflon septa. The first and
second water samples (day 0 and day 1) were taken 5 min after water
exchange in order to verify that our stock solution delivered to the
renewed tanks provided for similar initial conditions for each 24 h
period. The last water sample (day 2, no water exchange) was taken 5 min
before fish were removed to estimate the degradation rate using a
firstorder decay. All water samples were stored at 4C for later chemical
analyses by the USEPA Region 8 Laboratory. Samples arrived at the USEPA
within 72 h and upon arrival were stored at -20°C until they were
extracted at 96 h. The No Observed effect concentration (NOEC) value of
test chemical in fish in a 48 hr study on the basis of mortality effect
was determine to be 0.0014 mg/L.
96 hr short term toxicity study was
conducted to determine the lethal effect of test chemical in
Oncorhynchus mykiss. The Lethal effect concentration (LC50) value of
test chemical in Oncorhynchus mykiss in a 96 hr study on the basis of
mortality effect was determine to be 0.18 mg/L. Thus based on the LC50
value, chemical consider to be toxic and consider to be classified as
aquatic acute 1 as per the CLP classification criteria.
In the fourth study from authoritative
database short term toxicity of test chemical were studied on fish
Ictalurus punctatus. Fish exposed with the chemical for 48 hrs and
performed under the static system. Based on the mortality of test fish
Ictalurus punctatus by the chemical after the exposure period of 48 hrs,
the LC50 was determine to be 0.14 mg/l. Thus based on the LC50 value, it
is concluded that the substance will exhibit toxicity to fish and
classified in aquatic acute 1 category.
Similar study was conducted to
determine the effect of test chemical on fish Pimephales promelas. After
the exposure period of 96 hr study of test chemical on Pimephales
promelas, no effect were observed (NOEC) at 0.054 mg/l on basis of
effect on behavior of fish.
Thus based on the overall studies,
chemical consider to be toxic and classified in aquatic acute 1 category
as per the CLP classification criteria.
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