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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to
Guideline:
other: Refer below principle
Principles of method if other than guideline:
Long term toxicity of test chemical were studied and determine on the reproduction of daphnia magna.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
Test type:
not specified
Water media type:
freshwater
Total exposure duration:
21 d
Reference substance (positive control):
not specified
Key result
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
0.005 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Other details not known
Validity criteria fulfilled:
not specified
Conclusions:
The LOEC was observed to be 0.0047 mg/L for 21 days based on the effect on reproduction.
Executive summary:

Long term toxicity of test chemical were studied and determine on the reproduction of daphnia magna. The LOEC was observed to be 0.0047 mg/L for 21 days based on the effect on reproduction.

Description of key information

Long term toxicity of test chemical were studied and determine on the reproduction of daphnia magna. The LOEC was observed to be 0.0047 mg/L for 21 days based on the effect on reproduction.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.005 mg/L

Additional information

Based on the various long term studies available for the test chemical, studies were reviewed to determine the toxic nature of test chemical on the mobility and reproduction of aquatic invertebrates. The studies are as mentioned below:

 

In the first long term toxicity of test chemical were studied and determine on the reproduction of daphnia magna. The LOEC was observed to be 0.0047 mg/L for 21 days based on the effect on reproduction.

 

Above study was supported by the below study from peer reviewed journal. 8 days long term toxicity studies were conducted to test the effect of chemical on ceriodaphnia .Five concentrations and blanks were prepared with dechlorinated tap water for each antimicrobial test, and the survival and the number of neonates was counted for eight days, when most of the Ceriodaphnia had finished delivering three broods. The solutions were replaced every 48 h (i.e. a semi-static system) and the concentration before and after the replacement was analyzed using the HPLC. Only the numbers of first three broods were counted and compared with the blanks. Multiple comparisons were conducted to determine NOEC values as presented above for the algal test. After the exposure period of 8 days of chemical with Ceriodaphnia, no effect were observed on the survival rate and reproduction rate of Ceriodaphnia at the concentration 0.0019 mg/l. Thus based on the NOEC value chemical consider to be toxic and classified as aquatic chronic 1 as per the CLP classification criteria.

 

Similar long term toxicity of test chemical were studied on Americamysis bahia. Test conducted under the flow- through system for 28 days. The effective concentration (EC50) value of test chemical in Americamysis bahia(Opossum Shrimp) in a 28 days study based on growth was observed to be > 0.001 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance, it is concluded that test chemical could exhibit short term toxicity to aquatic invertebrate (Americamysis bahia) and classified in Aquatic chronic category 1.

 

 

Aim of this study was to determine the effect of test chemical on the aquatic invertebrates. Test conducted under the flow-through system in which effect of chemical were tested by observing the effect of chemical on mortality and survival rate of daphnia magna. The Low observed effect concentration (LOEC) of test chemical on Daphnia magna in 21 days study on the basis of effect on mortality was determine to be 0.05 mg/L.

 

In the fifth study from secondary source, 21 days long term toxicity studies were conducted to test the effect of test chemical on daphnia magna. The NOEC was observed to be 0.0029 mg/L for 21 days based on the effect on reproduction.

Thus based on the overall studies from peer reviewed journal, authoritative database and secondary sources, chemical consider to be toxic and classified as aquatic chronic 1 as per the CLP classification criteria.