Zinc bis(dibutyldithiocarbamate)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

GLP-compliant comparable to guideline study, available as unpublished report, no restrictions, adequate for assessment.

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

Differs from current testing guidelines by using abraded skin surface.

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Zinc bis(dibutyldithiocarbamate)

EC Number:

205-232-8

EC Name:

Zinc bis(dibutyldithiocarbamate)

Cas Number:

136-23-2

Molecular formula:

C18H36N2S4Zn

IUPAC Name:

zinc bis(dibutyldithiocarbamate)

Details on test material:

BUTASAN, off—white powder
Purity: 99.9%

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Isf:(NZW), Isaac’s Farm, Litchfield, Illinois)
- Age at study initiation: Young adult
- Weight at study initiation: 2.39—2.86 kilograms
- Housing: one per cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: Animals were quarantined for at least five days.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

physiological saline

Details on dermal exposure:

TEST SITE
- % coverage: 10—30% of total body surface
- Type of wrap if used: The test material was held in place by means of an occlusive wrap of latex rubber secured by bandaging and elastic tape.


REMOVAL OF TEST SUBSTANCE
- The occlusive wrap was removed after approximately 24 hours and the excess material was wiped from the animal.

Duration of exposure:

24 hours

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5 males and 5 females per dose

Control animals:

not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Clinical observations were made three times during the first eight hours following test material administration and twice daily (morning and afternoon) thereafter until sacrifice, with the exception of one day when observations were only recorded once.
Body weights were recorded on days 0 (day of exposure), 7, and 14.
- Necropsy of survivors performed: yes

Statistics:

No data

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No deaths were observed in animals of either sex.

Clinical signs:

other: Erytherna was observed in the exposed area of three male animals and one female rabbit on the first day after exposure. No other clinical abnormalities were noted.

Gross pathology:

At necropsy, pale renal coloration was observed in one male animal, but this was not considered to be related to exposure to the test material.
No abnormalities were observed in the remaining nine rabbits.