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EC number: 205-232-8
CAS number: 136-23-2
For the column elution procedure, a
limit of detection value of <0.1 mg/L was observed for each fraction.
Because of this, no additional runs at lower flow rates were required.
Collected fractions were checked for
the Tyndall effect by turbidity measurements to ensure that eluted
fractions were effectively filtered and did not give significant light
scattering indicated presence of particles. Results were found to be
satisfactory as no significant differences were observed between the
buffer blank and the test item solutions.
It is noted that inconsistent spectra
were obtained for the test item solutions. None of the test item
solution spectra were consistent with the spectra of the test substance
standard and were therefore not considered to contain the test substance
above the calculated limit of detection of the method (0.1 mg/L).
The buffer blank spectrum (after
extraction with dichloromethane as for test item solutions) is
consistent with the test item solution spectra with regard to absorbance
profile although varying absorbance’s are observed. This suggests that
the buffer is contributing to the absorbance profile observed for the
test item solutions after extraction with dichloromethane.
The test solution absorbance’s have
therefore been corrected for the absorbance from the buffer extract
The standard 1B (Approximately 0.5
mg/L) spectrum indicates that the absorbance’s obtained for the test
item solutions and corrected for the absorbance of the buffer blank
extract would be below that of the lower test substance standards
prepared and are consistent with a concentration of < limit of detection
The standard 1D (approximately 2 mg/L)
spectrum indicates that the test substance absorbance profile is
different to that observed from the buffer extract blank and test item
The example spectra indicate the
variation observed for the test item solutions. Note that the extract
blank has the highest absorbance, indicating that the absorbance profile
obtained for the test item solutions is not consistent with that of
butyl zimate and therefore the test item solutions are not considered to
contain butyl zimate above the calculated limit of detection of the
The water solubility was determined to be < 0.1 mg/L at 25 °C and pH 8, OECD TG 105, column elution method
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