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EC number: 204-815-4
CAS number: 126-97-6
thioglycolate was tested in an in vitro gene mutation assay using
mammalian cells cultures both in the absence and presence of metabolic
activation (S9 mix), according to the Directive 2000/32/EC, B17 and in
compliance with the Principles of Good Laboratory Practice.
mammalian cell gene mutation assay (TK+/-), mouse lymphoma L5178Y cells
cultured in vitro were exposed to ammonium
thioglycolate (purity 71.1%) in
deionised water. Two parallels culture were used. The first main
experiment was performed without microsomal activation at concentrations
of 0, 13, 50, 100,
200, 400, 800 and 1600 µg/ml, and with activation at concentrations of 0,
50, 100, 200, 400,800 and 1600 µg/ml
a treatment period of 4 h. The second experiment was solely performed in
the absence of metabolic activation at concentrations of 0, 13, 25, 50,
100, 200, 400 and 600 µg/ml, with a treatment period of 24 h. Appropriate
positive controls were used and showed a statistical increase in mutant
colonies, indicating that the tests were sensitive and valid.
After a 48
rest period, cells were
incubated mutagenicity evaluation with trifluorothymidine (TFT).
substantial and reproductible dose dependant increase in mutant colony
numbers was observed in both main experiments. No relevant shift of the
ratio of small versus large colonies was observed up to the maximal
concentration of the test item.
these experimental conditions, ammonium thioglycolate did not induce any
increase in mutant colonies and is not considered as mutagenic in this
mouse lymphoma mouse.
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