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EC number: 204-815-4
CAS number: 126-97-6
The validation criteria for the control respiration rates and reference material EC50 values have been satisfied.- the two control respiration rates are within 15% of each other.- the EC50 (3-Hour contact time) for 3,5-dichlorophenol lies within the range 5 to 30 mg/l.
A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 209 "Activated Sludge, Respiration Inhibition Test", EEC Commission Directive 87/302/EEC and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.6800.Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous solution of the test material at concentrations of 3.2, 10, 32, 100, 320 and 1000 mg active ingredient (ai)/l for a period of 3 hours at a temperature of 21°C with the addition of a synthetic sewage as a respiratory substrate.The rate of respiration was determined after 30 minutes and 3 hours contact time and compared to data for the control and a reference material, 3,5-dichlorophenol.Results. The effect of the test material on the respiration of activated sewage sludge gave a 3-Hour EC50 of greater than 1000 mg ai/l*. The No Observed Effect Concentration (NOEC) after 3 hours exposure was 32 mg ai/l.The reference material gave a 3-Hour EC50 value of 11 mg/l.
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