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EC number: 218-485-4
CAS number: 2162-73-4
this in vitro assessment of the mutagenic potential of TRIDI
according to OECD 471 under GLP, tryptophan-dependent mutants of
Escherichia coli, strain WP2 uvrA (pKM101), were exposed to TRIDI
diluted in ethylene glycol dimethyl ether (EGDE). EGDE
was used as the vehicle control and untreated controls were also
independent mutation tests were performed in the presence and absence of
liver preparations (S9 mix) from rats treated with phenobarbital and
first test was a standard plate incorporation assay; the second included
a pre-incubation stage.
of TRIDI up to 5000 µg/plate were tested. This
is the standard limit concentration recommended in the regulatory
guidelines that this assay follows. Other
concentrations used were a series of ca. half-log10 dilutions of the
signs of toxicity towards the tester strain were observed in either
mutation test following exposure to TRIDI. Precipitate
was observed on all plates containing TRIDI at 1500 and 5000 µg/plate in
the absence of S9 mix and at 5000 µg/plate in the presence of S9 mix in
evidence of mutagenic activity was seen at any concentration of TRIDI in
either mutation test.
concurrent positive controls verified the sensitivity of the assay and
the metabolizing activity of the liver preparations. The
mean revertant colony counts for the vehicle and untreated controls were
within or close to the current historical control range for the
was concluded that TRIDI showed no evidence of mutagenic activity in
this bacterial system under the test conditions employed.
30 % S-9
investigated using the Salmonella/microsome test for point mutagenic
effects in doses up to 5000 µg per plate on four Salmonella
typhimurium LT2 mutants. These comprised the histidine-auxotrophic
strains TA 1535, TA 100, T 1537 and TA 98.
The study was performed according to the
OECD Guideline 471 with deviations (only 4 Salmonella strains tested)
and considered to be of good quality (reliability Klimisch 2). 8 µg per
plate did not cause any bacteriotoxic effects: Total bacteria counts
remained unchanged and no inhibition of growth was observed. At higher
doses, the substance had a weak, strain-specific bacteriotoxic effect.
Substance precipitation occurred at the dose 500 µg per plate and above.
Therefore this range could only be used to a limited extent up to 5000
µg per plate for assessment purposes.
Evidence of mutagenic activity of
triisopropyldiisocyanatobenzene was not seen. No biologically relevant
increase in the mutant count, in comparison with the negative control
The positive control sodium azide,
nitrofurantoin, 4 -nitro-1,2-phenylene diamine and 2-aminoanthracene had
a marked mutagenic effect, as was seen by a biologically relevant
increase in mutant colonies compared to the corresponding negative
was considered to be non-mutagenic without and with S9 mix in the plate
incorporation as well as in the preincubation modification of the
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