Reaction mass of Dimethyl [3-[(hydroxymethyl) amino]-3-oxopropyl] phosphonate and Dimethyl (3-amino-3-oxopropyl)phosphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-12-05 till 1992-02-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

Deion. water instead of dest. water for the from the stock solutions - Preparation of the inoculum: higher sludge weight after the washing procedure (sludge concentration in the test according to the guidelines) - 4 instead of 5 test concentrations

GLP compliance:

yes

Specific details on test material used for the study:

Test substance: DMPPA_701-402-5
Stability: guaranteed by the sponsor
Solubility: >50 g/l in water
Storage: room temperature
Appearance: liquid

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Deion. water

Test organisms (species):

activated sludge

Details on inoculum:

Test organism: communal culture of bacteria (ARA-Pro Rheno Basel)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Nominal and measured concentrations:

320, 100, 32 and 10 mg/l

Details on test conditions:

Experimental conditions:
Temperature: 20.4 °C
Dry sludge: 1.56 g/l
Duration: 3 h
Estimation technique: (O2-consumption, measured with an oxygen-sensitive electrode system: OXI DIGI 530 (SAV: 00 109 01).

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

No inhibition was seen at any of the test concentrations after 3h exposure.

Validity criteria fulfilled:

yes

Conclusions:

The IC50 was found to be >320 mg/L in this activated sludge respiration inhibition test.

Executive summary:

The toxicity of DMPPA_701-402-5 to microorganisms is assessed in a study conducted according to OECD Guideline 209. The concentrations tested were 320, 100, 32 and 10 mg/L. However no inhibition was seen at any of the test concentrations after 3h exposure. The IC50 of the reference substance 3,5-Dichlorophenol was equal to 10.0 mg/L (Criteria: 5-30 mg/L). In conclusion, the IC50 was found to be >320 mg/L in this activated sludge respiration inhibition test.

Description of key information

The IC50 was found to be >300 mg/L in this activated sludge respiration inhibition test.

Key value for chemical safety assessment

EC50 for microorganisms:

320 mg/L

Additional information

The toxicity of DMPPA_701-402-5 to microorganisms is assessed in a study conducted according to OECD Guideline 209. The concentrations tested were 320, 100, 32 and 10 mg/L. However, no inhibition was seen at any of the test concentrations after 3h exposure. The IC50 of the reference substance 3,5-Dichlorophenol was equal to 10.0 mg/L (Criteria: 5-30 mg/L). In conclusion, the IC50 was found to be >320 mg/L in this activated sludge respiration inhibition test.