Reaction mass of Dimethyl [3-[(hydroxymethyl) amino]-3-oxopropyl] phosphonate and Dimethyl (3-amino-3-oxopropyl)phosphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05.02.1992 - 19.10.1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST SYSTEM
The test organism was the species Daphnia magna. The daphnids were bred in the laboratories of the TF Product Ecology under standardized conditions. For the test, animals with an age of less than 24 hours were used. The quality of the daphnids will be checked four times per year by determining the EC50 values for potassium dichromate.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

136 mg/l CaCO3

Test temperature:

21 °C

pH:

7.7 - 7.8

Dissolved oxygen:

saturated at the start and 96 % at the end

Nominal and measured concentrations:

Based on a pretest, the test concentrations were chosen as 58, 100 mg/l nominal.

Details on test conditions:

The daphnids were exposed to the test article in 250 ml beakers containing 100 ml test medium with 10 animals per beaker. All tests are
running in duplicate. During exposure, the animals were kept at 21 ± 1 °C with 16 hours illumination.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 was considered to be >100 mg/L.

Executive summary:

DMPPA_701-402-5 was assessed for short term toxicity to Daphnia magna in a study conducted according to OECD Guideline 202 and EU Method C.2. The daphnids were exposed to the test article at 0, 58 and 100 mg/L in 250 ml beakers containing 100 ml test medium with 10 animals per beaker. All tests were run in duplicate. During exposure, the animals were kept at 21 ± 1 °C with 16 hours illumination. No immobilisation was seen at any of the test concentrations. Hence, the 48h EC50 was considered to be >100 mg/L.

Description of key information

The substance was found to be practically non-toxic to Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 600 mg/L

Additional information

DMPPA_701-402-5 was assessed for short-term toxicity to Daphnia magna in a study conducted according to OECD Guideline 202 and EU Method C.2. The daphnids were exposed to the test article at 0, 58 and 100 mg/L in 250 ml beakers containing 100 ml test medium with 10 animals per beaker. All tests were run in duplicate. During exposure, the animals were kept at 21 ± 1 °C with 16 hours illumination. No immobilisation was seen at any of the test concentrations. Hence, the 48h EC50 was considered to be >100 mg/L. In a second valid study without GLP testing higher concentrations of the test item to Daphnia magna, the 48h-EC50 value of 1600 mg/L was estimated.