Cyclooctanone

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Remarks:

developmental toxicity

Type of information:

experimental study

Remarks:

publication

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Effects of external chemical environment on the developing olfactory bulbs of the mouse (mus musculus)

Author:

Harvey FE, Cowley JJ

Year:

1984

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Female mice were reared and observated from day 1 of life for three weeks. They were continously exposed to the odors of either cyclooctanone, adult male mouse urine or distilled water as control. Body weight, vaginal opening and first oestrus, olfactory preference, behavior and histological analysis of olfactory bulbs was recorded.

GLP compliance:

no

Type of method:

in vivo

Test material

Test material information

Constituent 1

Reference substance name:

Cyclooctanone

EC Number:

207-940-2

EC Name:

Cyclooctanone

Cas Number:

502-49-8

Molecular formula:

C8H14O

IUPAC Name:

cyclooctanone

Specific details on test material used for the study:

cyclooctanone crystals (Koch-Light Laboratories, Bucks)

Test animals

Species:

mouse

Strain:

other: T.O. mice

Sex:

female

Administration / exposure

Route of administration:

inhalation

Vehicle:

unchanged (no vehicle)

Details on exposure:

odorants were placed in petri dishes below the wire mesh floor of the living area of the observation incubators and in the path of the airflow which was fed in from outside the building by a vacuum/pressure pump with an output of approximately 1 L/min. Odorants were placed in the incubators daily.

Duration of treatment / exposure:

from day 1 of life for three weeks

Frequency of treatment:

continously

Duration of test:

up to 8 months

Doses / concentrations

Dose / conc.:

0.25 other: g

Remarks:

giving an average molar concentration of 7.3 x10^-5 M

No. of animals per sex per dose:

23 per dose

Control animals:

yes, sham-exposed

Results and discussion

Observed effects

Body weigth: stat. sign. Increased on day 16, other days no differences

Vaginal opening: at day 26.45 (control at day 27.37)
First oestrus: at day 30.77 (control at day 30.89)

Behavior: No treatment effect on grooming; altered sniffing pattern over days but not generally increased

Histology of olfactory bulb: significantly wider olfactory bulbs, significantly greater overall number of altered mitral cells

Applicant's summary and conclusion

Conclusions:

Exposure to cyclooctanone via inhalation for the first three weeks of life leads to no difference in age of sexual maturation of female mice. Exposure during neonatal period of female mice showed cellular changes in olfactory bulbs but effects were not considered adverse. Behavior was not adversely affected by exposure to cyclooctenone.