Bis(D-gluconato-O1,O2)zinc

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

LCE11031, Batch 1106900005 (zinc gluconate)

Details on sampling:

At the beginning and the end ot test, aliquot (approximately 20 ml.) was taken in each group containing the test item and in the control.
These aliquots were frozen and transferred to ICP unit to be analyzed for Zinc contents determination.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
Stock solutions were kept at 4 ± 2°C until they were used for the study. Stock solutions were used because they were not cloudy (without any precipitation).
on March 29th , 2011, April 4th, 2011 and April 11th, 2011, 250 mL of stock solution 1, 2, 3, and 4 were mixed with 800 rnL demineralised water and completed to 10L.
Test medium was kept less than I week at 4 ± 2°C
Test medium was used on April 1st, 5th and 12th, 2011.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain: Daphnia magna (prescribed by test guideline)
- Source: SGS breeding lab
- Age of parental stock: Daphnids between 6 and 24 hours old and produced from parthenogenesis reproducing brood female population.
Every day, daphnids were filtered on the following sieves (200 pm, 560pm and 800prn). We used for the test, Daphnids which pass through 800 µm sieve and remain on 560 µm sieve.

ACCLIMATION
no detail provided in the study report

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

Standard duration

Hardness:

not specified

Test temperature:

20°C ± 2

pH:

7.8 ± 0.2

Dissolved oxygen:

> 7mg/L (90%)

Nominal and measured concentrations:

0mg/L (control) ; 2.0196 mg/L ; 4.0392 mg/L ; 8.0784 mg/L ; 16.1568 mg/L ; 32.3136 mg/L

Details on test conditions:

TEST SYSTEM
- Aeration: During the preparation, test medium was ventilated until a value close to saturation in air.

OTHER TEST CONDITIONS
- Light intensity: Test carried out in the dark

PARAMETERS MEASURED:
The pH was measured in the control and all test concentrations, in the fresh solutions and in the old solutions.
The dissolved oxygen concentration was measured in control and in the lowest concentration that produced the maximum effect.

RANGE-FINDING STUDY
- Test concentrations: 0.01 - 0.1 - 1 - 10 - 100 mg/L (divided into 4 replicates including 1 control replicate (medium only) and 5 daphnids introduced in each replicate)
- Results used to determine the conditions for the definitive study: 50% of mortality (average between replicates) is observed at 10.9 mg/L

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

22.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).

Reported statistics and error estimates:

Confidence limits (95%) 20.4-25.3 mg/L

LCE11031 (mg/L)	Mortality Daphnids at 48h				Mortality (%)
	Replicate A	Replicate B	Replicate C	Replicate D
Control	0	0	0	0	0
2.0196	0	0	0	0	0
4.0392	0	0	0	0	0
8.0784	0	0	0	0	0
16.1568	1	2	0	2	25
32.3136	3	4	3	5	75

5 mobile dphnids per replicate at t=0

Validity criteria fulfilled:

yes

Conclusions:

The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).

Executive summary:

The study is an acute toxicity test performed on Daphnia Magna according to the OECD 202 guidline and to GLP. This study is considered reliable and it is used for assessment. The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).

Description of key information

The study is an acute toxicity test performed on Daphnia Magna according to the OECD 202 guidline and to GLP. This study is considered reliable and it is used for assessment. The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

22.8 mg/L