Bis(D-gluconato-O1,O2)zinc

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

LCE11031, Batch 1106900005 (Zinc Gluconate) as supplied by Givaudan Lavirotte

Analytical monitoring:

yes

Details on sampling:

At the beginning, before and after renewal and at the end of test, aliquot (approximately 20mL) was taken in each group containing the test item and in the control.
These aliquots were transferred to ICP unit to be analyzed for Zinc contents determination.

Vehicle:

no

Details on test solutions:

Stock solutions were kept at 4±2°C until they were used for tha study. Stock solutions were used because they were not cloudy (without any precipitation).

On March 2011, 100 mL of stock solution 1, 2, 3, and 4 was mixed with 800 mL demineralised water and completed to 10L and 2L of stock solution 1, 2, 3, and 4 was mixed with 10L demineralised water and completed to 200L.
On April 29th, 2011, 1000mL of stock solution 1, 2, 3, and 4 was mixed with 8L demineralised water and completed to 100L. Test medium was ventilated until a value close to saturation in air and pH stabilization were obtained.

Validity criteria for test medium :
O2 dissolved > 7mg/L (90%).
pH= 7-8 ± 0.2

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: "pisciculture", 27400 Hondouville, France

ACCLIMATION
- Acclimation period: at least 12 day before the start of the test
- Acclimation conditions: same as test
- Type of food during acclimation: trout chow.
- Feeding frequency during acclimation: Twice daily. The feeding was stopped 24 hours before the start of the test.

FEEDING DURING TEST
- Food type: trout chow

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

Standard duration applied.

Hardness:

Not specified

Test temperature:

15°C ± 2°C

pH:

mean of 7.87 at 96h ; 7.82 at 72h ; 7.09 at 0h

Dissolved oxygen:

> 60% (mean of 83.77% at 96h ; 93.72% at 72h ; 93.59% at 0h)

Nominal and measured concentrations:

10 ; 20 ; 40 ; 60 ; 80 ; 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Aeration: Forced aeration to keep the dissolved oxygen concentration at or above of the saturation value at the selected temperature.
- No. of organisms per concentration: 7 fish in each concentration

OTHER TEST CONDITIONS
- pH: regular pH monitoring
- Light photoperiod cycle: 8/16 hours.

TEST CONCENTRATIONS
- Range finding study test concentration: 0.01 - 0.1 - 1 - 10 - 100 mg/L
- Test concentrations (definitive test): 10 - 20 - 40 - 60 - 80 and 100 mg/L

OTHER :
After 48 hours, news solutions were prepared and living fish were transferred into them.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

11.12 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).

Reported statistics and error estimates:

confidence limits (95%): 10-10mg/L

Validity criteria fulfilled:

yes

Conclusions:

The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).

Executive summary:

An OECD 203 study (GLP) was conducted on Oncorhynchus mykiss under semi-static conditions. This study is considered reliable. The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).

Description of key information

An OECD 203 study (GLP) was conducted on Oncorhynchus mykiss under semi-static conditions. This study is considered reliable. The EC-96h determined on Oncorhynchus mykiss is 11.12mg/L (confidence limits (95%): 10-10mg/L).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

11.12 mg/L