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EC number: 281-689-7
CAS number: 84012-44-2
Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Triticum aestivum, Gramineae.
[The objectiveothe study was
to evaluate WICKENOL 535 for its ability to generate toxic effects inRaphidocelis
subcapitata(formerly known asPseudokirchneriella subcapitata)during
an exposure period of 72 hours and, if possible, to determine the NOEC,
EC10and EC50for both inhibition of growth rate and
inhibition of yield.
The study procedures described in this report were based on the
OECD guideline No. 201, 2006; Annex 5 corrected 28 July 2011. In
addition, procedures were based on the test methods described in the
OECD series on testing and assessment number 23, 2000.
The batch of WICKENOL 535 tested was astraw to yellow
syrup. The test item was a UVCBwhich was not completely soluble in
test medium at the loading rates initially prepared.
For the combined limit/range finding test and first full test,Water
Accommodated Fractions (WAFs) were individually prepared at loading
rates ranging between 1.0 mg/L and 100 mg/L and used as test
concentrations. For the second full (and final) test, a stock solution intetrahydrofuran
(THF)was prepared at a concentration of 5 mg/ml. Lower
stock solutionswere prepared separately by proportional dilution of the
highest stock solution in THF. For the preparation of each test
concentration, 100 µL of each of the stock solution was added per 1 L of
A second full (and final) test was performed based on the results
of a preceding combined limit/range-finding test and a first full test.
Six exponentially growing algal cultures were exposed to an untreated
control and to a solvent control, whereas three replicates per group
were exposed to 0.023, 0.050, 0.11, 0.23 and 0.50 mg WICKENOL 535 per
litre. The initial algal cell density was 104cells/mL.The
total exposure period was 72 hours and samples for analytical
confirmation of actual exposure concentrations were taken at the start,
after 24 and 72 hours of exposure.
No statistical difference was recorded between the blank control
and the solvent control. No significant differences were recorded
between the values for growth rates at any of the test concentrations
when compared to the pooled control groups. Inhibition of yield was
statistically significant but biological not relevant (i.e., <10%) at
the three highest test concentrations when compared to the pooled
Samples taken from all test concentrations and the control were
analysed. The initial measured concentrations were 0.012, 0.028, 0.081,
0.18 and 0.43 mg/L, respectively, in 0.023, 0.05, 0.11, 0.23 and
0.50 mg/L. Measured concentrations decreased already within 24 hours to
values below the limit of detection or to values that could only be
estimated. Consequently, as no significant biological effects were
observed, a TWA concentration of0.0089 mg/L was calculated for the
highest test concentration.
The study met the acceptability criteria prescribed by the study
plan and was considered valid.
The effect parameters obtained in this study are summarized in the
* - based on statistical significance,
** - based on biological relevance
Due to the very low solubility of WICKENOL 535 in water,
concentration levels that might be toxic for algae could not be reached.
paste the summary of the report]
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