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EC number: 281-689-7
CAS number: 84012-44-2
Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Triticum aestivum, Gramineae.
The objective of the study was to evaluate WICKENOL 535 for its
ability to generate acute toxic effects on the mobility of Daphnia
magna during an exposure period of 48 hours and, if possible, to
determine the EC50at 24 and 48 hours of exposure.
The study procedures described in this report were based on the
OECD guideline No. 202, 2004.In addition, procedures were based on the
test methods described in the OECD series on testing and assessment
number 23, 2000.
The batch of WICKENOL 535 tested was astraw to yellow syrup. The
test item was a UVCB which wasnot completely soluble in test medium at
the loading rates initially prepared.
A combined limit/range-finding test was performed. Water
Accommodated Fractions (WAFs) were individually prepared at loading
rates of 1.0, 10 and 100 mg/L and used as test concentrations.
No biologically relevant immobility or other effects were observed
in the control and at any of the concentrations tested.
Samples taken from the limit concentration were analysed. The
measured concentration was 3.1 mg/L at the start of the test. This
concentration decreased to 0.19 mg/L at the end of the exposure. Based
on these results, effects parameters were based on the average exposure
concentration of 0.78 mg/L.
The study met the acceptability criteria prescribed by the study
plan and was considered valid.
In conclusion, the 48h-EC50forDaphnia magnaexposed
to WICKENOL 535 exceeded an average exposure concentration of 0.78 mg/L,
being considered the maximum soluble concentration of the test item in
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