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EC number: 281-689-7
CAS number: 84012-44-2
Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Triticum aestivum, Gramineae.
The objective of the study was
to evaluate the test item WICKENOL 535 for its ready biodegradability in
an aerobic aqueous medium with microbial activity introduced by
inoculation with activated sludge.
The study procedures described in this report
were in compliance with the OECD guideline No. 301 B, 1992. At the
request of the Sponsor, the test item was coated on silica particles.
Additional blanks were included to correct for the possible effects of
WICKENOL 535 was a straw to yellow syrup
(UVCB) and was not sufficiently soluble to allow preparation of an
aqueous solution at a concentration of 1 g/L. The Total Organic Carbon
(TOC) content of the test item was determined to be 76.23%. Based on the
TOC content the ThCO2of the test item was calculated to be
2.80 mg CO2/mg. The test item was tested in duplicate at
a target concentration of 15.7 mg/L, corresponding to 12 mg TOC/L.
The study consisted of eight bottles:
inoculum blanks (no test item),
silica blanks (silica, no test item),
test bottles (silica, WICKENOL 535),
procedure control (sodium acetate) and
toxicity control (silica, WICKENOL 535, and sodium acetate).
Aliquots of 37 µL were pipetted directly onto weighed amounts of
silica in clear glass weighing bottles. After careful mixing, the silica
containing the test item was added directly to the 2 litre test bottles
containing medium with microbial organisms and mineral components.
Subsequently, Milli-RO water was added to each weighing bottle
containing the silica in order to quantitatively add all silica to the
test medium.The test solutions were
continuously stirred during the test, to ensure optimal contact between
the test item and the test organisms.Test
duration was28 days for the inoculum blank, silica blank,
and test item (last CO2measurement on day 29) and 14 days for
the procedure and toxicity control (last CO2measurement on
The relative biodegradation values calculated
from the measurements performed during the test period revealed 66% and
53% biodegradation of WICKENOL 535, for A vessel and B, respectively
(based on ThCO2). Furthermore, since the test item is a
mixture of structurally similar chemicals and gradual biodegradation is
anticipated, the 10-day window need not be applied.
In the silica blank, slightly higher
background CO2production was observed. The test item bottles
were compared with the silica blanks.
In the toxicity control, WICKENOL 535 was
found not to inhibit microbial activity.
Since all criteria for acceptability of the
test were met, this study was considered to be valid.
the results of the present test indicate that the pass level criterion
was almost fulfilled for bottle B the results can be used to indicate
based on theresults of vessel A,WICKENOL
535 can be considered readily biodegradable under the conditions of the
modified Sturm test presently performed.
Guidelines For The Testing Of Chemicals. Revised Introduction To The
OECD Guidelines For Testing Of Chemicals, Section 3, Part 1, Chapter
2.5, Paragraph 36. (adopted July 23 March 2006).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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