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EC number: 624-182-1 | CAS number: 4187-38-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 June 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- D-α-methylbenzylamine
- EC Number:
- 223-423-4
- EC Name:
- D-α-methylbenzylamine
- Cas Number:
- 3886-69-9
- Molecular formula:
- C8H11N
- IUPAC Name:
- 1-phenylethanamine
- Details on test material:
- - Name of test material (as cited in study report): (R)-(+)-1-Phenylethylamin
- Substance type: active substance
- Physical state: liquid
- Analytical purity: 99.6 area-% (analytical report: ZAX 98L00289)
- Lot/batch No.: 68-0655 (date of production: 09 Feb. 1997)
- Stability under test conditions: instable against acids and alkali
- Storage condition of test material: room temperature
- Other:
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The substance was added directly to the test vessels.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: yes, municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated
for 24 hours. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20±2 °C
- Dissolved oxygen:
- Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement of oxygen consumption: > 6.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel
- Material, size, headspace, fill volume: glass, nominal volume: 250 mL, fill volume 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
OTHER TEST CONDITIONS
- Adjustment of pH: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 550 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid?: yes
- Relevant effect levels: 30-min EC20: ca. 1.8 mg/L; 30-min EC50: ca. 6.7 mg/L; 30-min EC80: ca. 60 mg/L
Any other information on results incl. tables
Respiration data:
Treatment |
BC1 |
BC2 |
BC3 |
BC (mean value) |
TS1 |
TS2 |
TS3 |
RS1 |
RS2 |
RS3 |
Concentration of test substance (mg/L) |
- |
- |
- |
- |
1000 |
500 |
250 |
1 |
10 |
100 |
O2 consumption rate (mg O2/L*h) |
30 |
28 |
28 |
29 |
18 |
24 |
26 |
27 |
12 |
4 |
Specific O2 consumption rate (mg O2/g*h) |
30 |
28 |
28 |
29 |
18 |
24 |
26 |
27 |
12 |
4 |
Change in oxygen consumption rate compared to blank control (%) |
- |
- |
- |
- |
-38 |
-17 |
-10 |
-7 |
-59 |
-86 |
pH value* |
6.4 |
6.5 |
6.6 |
|
9.8 |
9.6 |
8.9 |
6.5 |
6.6 |
6.5 |
* The pH value has been measured and adjusted to pH 7.0 to 8.0 before the addition of activated sludge.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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