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EC number: 271-089-3
CAS number: 68515-47-9
Cytotoxicity ranged from 3.5 - 21 % at the high dose levels. In the
absence of activation, 2 to 10 ul/ml induced moderate to high toxicity
(percent relative growths: 18.8% to 70.7%), but no increase in mutation
frequency. In the presence of a metabolic fraction, 1 to 8 ul/ml was
toxic (percent relative growths: 3.5% to 62.7%), without increasing the
incidence of mutations. Thus, the test compound was considered
non-mutagenic with activation in this assay.
In a mammalian cell gene mutation assay at the thymidine kinase locus,
L5178Y mouse lymphoma cells cultured in vitro were exposed to the test
substance, in acetone, at concentrations of 2.0, 4.0, 5.0, 6.0, 8.0,
or 10.0 ul/ml in the absence of S9 mammalian metabolic activation or
at concentrations of 1.0, 2.5, 4.0, 5.0, 5.54, or 8.0 ul/ml in the
presence of S9.
The test substance was tested up to cytotoxic concentrations. Under
non-activation conditions (dose levels 2 -10 ul/ml), the percent
relative growth values were 70.7%-18.8%, indicating moderate to high
toxicities, and the mutant frequencies were18.1 x 10 -6 to 30.7 x 10
-6. Because moderate to high toxicity was observed, but mutagenesis
was not observed at any dose level assayed, the test substance was
determined not to be a mutagen under non-activation conditions. In the
S9 activation assay, the test substance was assayed at low to highly
toxic dose levels (1 -8 ul/ml), and the mutant frequencies for the
assayed dose levels were 21 x 10^-6 to 53.9 x 10^-6. The test
substance was determined not to be a mutagen under S9 activation
conditions. In both the non-activated and activated conditions, the
positive controls induced the appropriate response. There was no
evidence of induced mutant colonies over background as a result of
exposure. The test material is considered inactive in the mouse
lymphoma forward mutation assay with and without metabolic
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