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EC number: 213-022-2
CAS number: 915-67-3
Table 1: Mutagenicity of food colours in
tester strains of Salmonella typhimurium
Mean of the No. Revertant Colonies ± S.D.
222.0 ± 49.79
178.3 ± 30.29
827.70 ± 106.53
522.0 ± 50.48
SA= Sodium azide
S.D.= Standard Deviation
Anova Value of TA97a (Amaranth —2.11, ns)
TA 98 (Amaranth –10.19*)
TA100 (Amaranth —2.196, ns)
Ames mutagenicity assay was performed
to evaluate the mutagenic nature of the test compound Amaranth in plate
The test material was tested at a
concentration of 10,100,250,500 and 1000 μg /plate. The plates were
inverted within an hour and placed in a dark vented incubator at 37⁰C
for 48 hours. Positive controls (for TA97a and TA98, 20 μg/plate nitro
phenylene diamine and for TA100, 1.5 μg/plate sodium azide) and negative
controls were maintained concurrently for all the experiments. Three
plates were used for each set. After 48 hours of incubation, the
revertant colonies were counted. ANOVA test was performed at 0.05 level.
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