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EC number: 295-518-9
CAS number: 92062-05-0
A complex combination of hydrocarbons obtained from the vacuum distillation of the products from a thermal cracking process. It consists predominantly of hydrocarbons having carbon numbers predominantly greater than C34 and boiling above approximately 495°C (923°F).
across in vivo micronucleus test on oxidised bitumen was negative for
genotoxicity. In addition chronic inhalation studies with oxidised
bitumen, together with comparative fume compostion information, indicate
that read across to the bitumen category, is appropriate.
In a mammalian
micronuclei assay, Chinese hamster cell V79 cultures were exposed to
Type I and Type III Asphalt Fume Condensates in DMSO at concentrations
of 0, 62.5, 125, 187.5, and 250 μg/mL for 24 hours.
Cells were subcultured at a concentration of
5 x 105 cells/ 60 mm dish for each treatment. Incubation
Period: Cells were incubated at 37 degrees Celsius in a 5% carbon
dioxide humidified atmosphere for 24 hours. The asphalt fume condensate
dissolved in DMSO was added to each culture. Four different
concentrations were tested for each sample. Cells were treated for 24
hours, then washed twice with PBS, and fresh medium was placed in each
disk. After an additional 24-hr incubation, cells were detached by
trypsinization, collected by centrifugation, and resuspended in culture
medium at a density of 1 x 106 cells/mL.
The results were expressed as the mean
number of cells with micronucleus per 1000 cells. Both types of Asphalt
Fume Condensates were found capable of causing micronucleaus formation
in mammalian cells in vitro. The genotoxic potential appears to be
similar for both types of asphalt condensate. Both condensates caused a
similar dose-related increase in the frequency of micronucleated cells.
The increase was statistically significant for all four concentrations
tested. These findings indicate that both Type I and Type IIl roofing
asphalt fumes are capable of causing principally cytogenetic damage by
spindle apparatus in cultured mammalian cells.
This study received a
Klimisch score of two and is classified as reliable with restrictions because
it is an acceptable and a well-documented study report.
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