1-Butanol, 3-amino-, (3R)-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

2013-09-09 - 2013-09-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Justification for data waiving:

other:

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2867-59-6

Cas Number:

2867-59-6

IUPAC Name:

2867-59-6

Constituent 2

Reference substance name:

3-Amino-butan-1-ol

IUPAC Name:

3-Amino-butan-1-ol

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): 3-Amino-butan-1-ol
- Physical state: Liquid/ colourless, clear
- Analytical purity: 93.6 corr. area-%.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Laboratory culture: Activated sludge from the municipal wastewater treatment plant of Mannheim, Germany was collected from the aeration tank of the plant
- Preparation of inoculum for exposure: The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with tap water and the suspension was adjusted to 3 g/L dry matter.
- Pretreatment: None
- Initial biomass concentration: 1.5 g/L dry mass

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

180 min

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel: Glas-beakers (nominal volume 1000 mL)
- Test volume: 500 mL
- Aeration: incubation was started by aeration of the test vessels with pressure air
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):6
- No. of vessels perreference substance concentration (replicates):2

OTHER TEST CONDITIONS
- Adjustment of pH: to 7.2 with 1 M solution

TEST CONCENTRATIONS
- Test concentrations: 62.5, 125, 250, 500, 1000 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

EC10

Effect conc.:

560 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

780 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

The EC50 of 3,5-dichlorophenol was 6.7 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes