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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2013-09-09 - 2013-09-10
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Justification for data waiving:
other:

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
2867-59-6
Cas Number:
2867-59-6
IUPAC Name:
2867-59-6
Constituent 2
Reference substance name:
3-Amino-butan-1-ol
IUPAC Name:
3-Amino-butan-1-ol
Test material form:
other: liquid
Details on test material:
- Name of test material (as cited in study report): 3-Amino-butan-1-ol
- Physical state: Liquid/ colourless, clear
- Analytical purity: 93.6 corr. area-%.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Laboratory culture: Activated sludge from the municipal wastewater treatment plant of Mannheim, Germany was collected from the aeration tank of the plant
- Preparation of inoculum for exposure: The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with tap water and the suspension was adjusted to 3 g/L dry matter.
- Pretreatment: None
- Initial biomass concentration: 1.5 g/L dry mass

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
180 min

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel: Glas-beakers (nominal volume 1000 mL)
- Test volume: 500 mL
- Aeration: incubation was started by aeration of the test vessels with pressure air
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):6
- No. of vessels perreference substance concentration (replicates):2

OTHER TEST CONDITIONS
- Adjustment of pH: to 7.2 with 1 M solution

TEST CONCENTRATIONS
- Test concentrations: 62.5, 125, 250, 500, 1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
EC10
Effect conc.:
560 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
780 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
The EC50 of 3,5-dichlorophenol was 6.7 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes