1-Butanol, 3-amino-, (3R)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guidline study

Justification for data waiving:

other:

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2867-59-6

Cas Number:

2867-59-6

IUPAC Name:

2867-59-6

Constituent 2

Reference substance name:

3-Amino-butan-1-ol

IUPAC Name:

3-Amino-butan-1-ol

Details on test material:

Identification: 3-Amino-butan-1-ol
Chemical name: 1-Butanol, 3-amino-
CAS: 2867-59-6
Physical form: liquid
Appearance: clear, colouless
Water solubility: miscible
Purity: 93.6 corr. area %
Molecular weight: 89.137 g/mol
Density: 0.9440 ± 0.0001 (20°C)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling at the start of the exposure (0h) was carried out immediately after preparation of the limit concentration (un-neutrolised and pH-neutrolised). At the end of the exposure (48h), samples were taken directly from the test vessels, which contained the daphinds.

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution (100 mg/l of the test item was weighed out) was freshly prepared with dilution water in a measuring flask. The limit concentration of 100 mg/l was initially adjsuted to pH 7 ± 0.2 by the addition of 1 M HCl and tested under the same conditions as an un-neutralised trial with a limit concentration of 100 mg/l. The limit concentration (un-neutralised and pH-neutralised) was selected based on the results of a non GLP preliminary range finding test conducted at the test facility under static conditions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age at study initiation (mean and range, SD):
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding: In glass vessels (2 -3 l capacity) with approximately 1.8 l culute medium at 20 ± 2°C, in an incubator, 16 h illumination; light intensity of max. 20µEm-2s-1
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

178 mg CaCO3/l

Test temperature:

18 - 22°C, constant within ± 1°C

pH:

7.26 - 10.05

Dissolved oxygen:

8.24 - 8.85 mg/l

Details on test conditions:

20 daphnids, divided into 4 replicates, each with 5 daphnids, were used for the limit concentration (un-neutralised and pH-neutralised) and the control. Per replicate, 20 g of each test solution was weighed out into the test vessel. This corresponds to 20 ml per test vessel. The daphnids were inserted with a small amount of dilution water by a pipette.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility