DODECANEDIOIC ACID, 1,12-BIS(2-OCTYLDODECYL) ESTER

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 Jan 2020 to 31 Jan 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test organisms (species):

Daphnia magna

Details on test organisms:

Juvenile Daphnia magna (Straus), less than 24 hours old, were taken from healthy parental laboratory cultures, held and maintained at the test facility. These cultures were set up originally from dormant eggs (ephippia) supplied by MicroBioTests Inc., Belgium.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

See table 1

pH:

See table 1

Dissolved oxygen:

See table 1

Details on test conditions:

Due to the low solubility of the test substance it was expected that there would not be a toxic effect at the limit of solubility therefore a definitive limit test was conducted at a nominal loading rate of 100 mg/L. A control group was also included.
The test vessels were 100 mL tall form glass beakers (filled to approximately 50 mL). Four replicate test vessels were prepared for the control and test concentration.
At the start of the test, 50.00 mg of test substance was weighed onto a glass weigh boat, this was then suspended below the water level of the Elendt M4 media fully submerging the test substance. Preparations were then allowed to stir slowly (such that a slight dimple no deeper than 1 cm was formed at the surface) for ca 48 hours, followed by a ca 1 hour settle period. Final media were then syphoned from the mid-section of the vessel or aqueous phase. The control was treated in the same manner without the addition of test substance.
Five juvenile Daphnia magna, less than 24 hours old, were added to each test and control vessel, using a wide bore glass pipette to avoid damaging the animals during transfer. The Daphnia magna were not fed during the test.
After 24 and 48 hours, the Daphnia magna in each test vessel were observed for evidence of immobility.
The observations differentiated between mobile and immobile daphnids. An individual was considered immobile if, when the contents of the test vessel were briefly agitated, it did not swim during a 15-second period of observation. In addition, Daphnia magna submerged in the body of the test media and those that were held at the surface of the test media were also recorded.

The test was conducted with a 16-hour light: 8-hour dark photoperiod.
The pH, dissolved oxygen concentration (% air saturation value (ASV) and mg/L) and temperatures were determined in freshly prepared test media at the start of the test and in the old media at 48 hours. Continuous temperatures were measured using a digital (min/max) thermometer in an additional vessel maintained in the test area.
The freshly prepared media water quality measurements were performed on media sampled directly from preparation flasks. The old media water quality measurements were conducted using pooled replicate samples of old test media.

Reference substance (positive control):

yes

Remarks:

Potassium Dichromate with 100% purity used as a test substance, experimental dates 10th December 2019-12 December 2019.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The 48-hour acute toxicity of Dioctyldodecyl Dodecanedioate to the freshwater planktonic crustacean, Daphnia magna, was determined in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).
As there was no valid analytical method available to measure the test substance concentration at the time of testing, the results were based on nominal loading rates.
Based on nominal loading rates, the 48-hour EC50 value was estimated to be >100 mg/L.
The corresponding NOEC value was 100 mg/L.
All validity criteria were satisfied, therefore the test was considered valid.

Conclusions:

The 48-hour acute toxicity of Dioctyldodecyl Dodecanedioate to the freshwater planktonic crustacean, Daphnia magna, was determined in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).
Based on nominal loading rates, the 48-hour EC50 value was estimated to be >100 mg/L.
The corresponding NOEC value was 100 mg/L.
All validity criteria were satisfied, therefore the test was considered valid.

Executive summary:

Product should not be classified according to CLP criteria for Acute or Chronic Hazards to the environment.

Description of key information

Based on result product should not be classified according to CLP criteria for Acute or Chronic Hazards to the environment.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L