Registration Dossier
Registration Dossier
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EC number: 603-333-5 | CAS number: 129423-55-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 February 2020 - 12 March 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Conducted to GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- Adopted 1984, Revised 1992
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- A sample of activated sludge was collected from one of the return lines at Harrogate South sewage treatment works (North Yorkshire, UK), which has a predominantly domestic waste-water catchment. The sample was transported in a closed container but with an adequate headspace, to prevent the sample becoming anaerobic. On arrival, the sample was aerated using a compressed air supply. The activated sludge used in this study was not acclimatised or adapted to the test substance prior to exposure under the test conditions.
- Duration of test (contact time):
- >= 28 - ca. 35 d
- Initial conc.:
- ca. 15 other: mg/L
- Based on:
- test mat.
- Remarks:
- Dioctyldodecyl Dodecanedioate was accurately weighed (28.5 mg) for direct addition to test substance and toxicity control vessels via a glass slide, to give a nominal test substance concentration corresponding to 15 mg carbon/L.
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Supplier: VWR chemicals; lot: 17EO24121; expiry: 31 August 2021
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 75
- Sampling time:
- 35 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 67
- Sampling time:
- 28 d
- Details on results:
- Dioctyldodecyl Dodecanedioate was not considered to have an inhibitory effect on the inoculum because the 25% biodegradation level (calculated on total theoretical carbon content of the vessel) was achieved in the toxicity control (containing the reference substance and Dioctyldodecyl Dodecanedioate) by Day 7. When calculated using the carbon content of the reference substance only (corrected for test substance degradation), this vessel showed a similar degradation pattern to the reference substance vessels.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- readily biodegradable, but failing 10-day window
- Executive summary:
readily biodegradable, but failing 10-day window
Reference
| Biodegradation (%) | ||
| Day | Test Item | Reference Substance |
| 2 | 0.08 | 29.89 |
| 5 | 1.78 | 49.9 |
| 7 | 12.81 | 59.62 |
| 9 | 24.2 | 63.51 |
| 12 | 34.76 | 65.95 |
| 16 | 45.21 | 70.4 |
| 19 | 53.91 | 75.37 |
| 21 | 58.14 | 77.65 |
| 23 | 60.8 | 79.62 |
| 26 | 64.29 | 82.31 |
| 28 | 66.56 | 83.55 |
| 33 | 69.84 | 84.62 |
| 35 | 72.37 | 85.28 |
| 36 | 75.03 | 86.25 |
Description of key information
Readily biodegradable but failing 10 day window: 67% (CO2 Evolution) in 28 days; 75% (CO2 Evolution) in 35 days
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable but failing 10-day window
- Type of water:
- freshwater
Additional information
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