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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)
Vehicle:
no
Details on test solutions:
Deionized water containing no more than 0.01 mg/L Cu and 1 mg/L nonpurgeable organic carbon was prepared in a water purification system.
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Unadapted secondary activated sludge was obtained (24-04-2012) from the WWTP Nieuwgraaf in Duiven. The WWTP Duiven is an activated sludge plant treating predominantly domestic wastewater. Prior to use the activated sludge was homogenized with a syringe. The dry weight of the homogenized activated sludge was determined and subsequently concentrated by settlement to the required dry weight concentration. The dry weight of the activated sludge in the incubation vessels was 1.5 g/L.
Test type:
static
Water media type:
freshwater
Total exposure duration:
3 h
Test temperature:
19.8 to 20.5 °C
pH:
7.2 to 7.4
Details on test conditions:
The test was performed in 300 mL Erlenmeyer flasks with a total working volume of 50 mL. The homogenized activated sludge was incubated in a shaking water bath (100 rpm, 20°C) for 3 hours with various concentrations of the test compound and synthetic sewage.
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
39 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
94 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
224 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Validity criteria fulfilled:
yes
Conclusions:
The EC50 of bis(pentane-2,4-dionato) zinc for activated sludge after 3 hours contact time is 94 mg/L.
The EC10, EC20 and EC80 are 25, 39 and 224 mg/L, respectively.
Executive summary:

The toxicity of bis(pentane-2,4-dionato) zinc to activated sludge was determined at a contact time of 3 hours, using various concentrations of the test substance. The inhibitory effect of bis(pentane-2,4-dionato) zinc at a particular concentration is expressed as a percentage of the two controls. From the results EC values were calculated. The EC50 of bis(pentane-2,4-dionato) zinc for activated sludge after 3 hours contact time is 94 mg/L with 95% confidence limits of 29 and 229 mg/L. The EC10, EC20 and EC80 are 25, 39 and 224 mg/L, respectively.

Description of key information

The EC50 of bis(pentane-2,4-dionato) zinc for activated sludge after 3 hours contact time is 94 mg/L.

The EC10, EC20 and EC80 are 25, 39 and 224 mg/L, respectively.

Key value for chemical safety assessment

EC50 for microorganisms:
94 mg/L
EC10 or NOEC for microorganisms:
25 mg/L

Additional information