[1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June to July 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Version / remarks:

Adopted 1981-05-12

Deviations:

not specified

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

[1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid

EC Number:

278-917-2

EC Name:

[1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid

Cas Number:

78479-03-5

Molecular formula:

C14 H14 Cl2 O2

IUPAC Name:

(1R,3S)-3-[2-chloro-2-(4-chlorophenyl)ethenyl]-2,2-dimethylcyclopropane-1-carboxylic acid

Test material form:

solid: crystalline

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: WINKELMANN, Borchen, Kreis Paderborn (Germany)
- Weight at study initiation: ca. 160-200 g
- Housing: Makrolon cages Type II (1 animal per cage during application and first week of observation), Low-dust wood pellets were used as litter.
- Diet: "Altromin 1324 - dietary diet for rats and mice" (producer: Altromin GmbH)
- Water (e.g. ad libitum): tap water.
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 +/- 2°C
- Humidity (%): 40 - 60 %
- Photoperiod (hrs dark / hrs light): 12 hours / 12 hours

Administration / exposure

Type of coverage:

occlusive

Vehicle:

physiological saline

Details on dermal exposure:

TEST SITE
- Area of exposure: back skin
- Type of wrap if used: the skin areas were covered with aluminum foils and fixed to the skin with the aid of wound dressings.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): water and soap.
- Time after start of exposure: 24 hours

VEHICLE
- physiological saline solution (0.9 % NaCl v/m)

Duration of exposure:

24 hours

Doses:

2.500 - 5.000 mg/kg bw

No. of animals per sex per dose:

5 males and 5 females per dose

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: The body weights of the rats were determined before application / exposure and afterwards weekly.
- Frequency of observations: the behavior and the appearance were determined several times on the application day / exposure day and thereafter twice per day.
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

female

Dose descriptor:

LD0

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

male

Dose descriptor:

LD0

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality at 5000 mg/kg bw for males and females

Clinical signs:

- Symptoms: Apathy and diminished mobility.
- Local findings: From 1 to 3 days after observation, small wounds and a slight hyperemia of the skin were observed at the application site.

Body weight:

In male rats, the body weight gain in the time after observation corresponded to the biological standard.
In female rats, a decreased body weight gain was observed (both groups).

Other findings:

Animals killed at the end of the observation: no evidence of substance-induced, macroscopically recognizable changes of the visceral organs.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

DL50 > 5000 mg/kg bw