Registration Dossier
Registration Dossier
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EC number: 278-917-2 | CAS number: 78479-03-5
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-05-13 - 2006-06-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Council Directive 92/69/EEC
This test method is in most parts equivalent to the OECD Guideline 201 (Alga, Growth Inhibition Test) - Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- [1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid
- EC Number:
- 278-917-2
- EC Name:
- [1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid
- Cas Number:
- 78479-03-5
- Molecular formula:
- C14 H14 Cl2 O2
- IUPAC Name:
- (1R,3S)-3-[2-chloro-2-(4-chlorophenyl)ethenyl]-2,2-dimethylcyclopropane-1-carboxylic acid
- Test material form:
- solid: crystalline
- Details on test material:
- From manufacturing at INTEROR's site
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: INTEROR S.A. / Lot INT 05 691
- Expiration date of the lot/batch: 2007-08-01
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: non-axenic strain
- Source (laboratory, culture collection): The Collection of Algal Cultures of the Institute of Plant Physiology at the University of Göttingen (Germany)
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21 - 25°C
- Nominal and measured concentrations:
- initial cell concentration: 0.5 x 104 cells/ml
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 ml Erlenmeyer flasks with cotton stoppers
- Initial cells density: 0.5x10E4 cells/ml
- Control end cells density: mean: 596667 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination is provided in the spectral range 400 to 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microcell counter
TEST CONCENTRATIONS
- Test concentrations: 100 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Analysis of the growth and growth rate of the algal population within the 72 h exposure period give the following results:
EbC 50 (0-72 h) > 100 mg/L
ErC 50 (0-72 h) > 100 mg/L
At the highest concentration of 100 mg/L growth rate was inhibited by 3.1 % compared to the control. Inhibition of biomass integral was 9.8 %.
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