[1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-03-17 - 2006-05-05

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Version / remarks:

Council Directive 92/69/EEC
This test method is in all essential parts identical with OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

[1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid

EC Number:

278-917-2

EC Name:

[1α,3β(Z)]-(±)-3-[2-chloro-2-(4-chlorophenyl)vinyl]-2,2-dimethylcyclopropanecarboxylic acid

Cas Number:

78479-03-5

Molecular formula:

C14 H14 Cl2 O2

IUPAC Name:

(1R,3S)-3-[2-chloro-2-(4-chlorophenyl)ethenyl]-2,2-dimethylcyclopropane-1-carboxylic acid

Test material form:

solid: crystalline

Details on test material:

From manufacturing at INTEROR's site

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: INTEROR S.A. / Lot INT 05 691
- Expiration date of the lot/batch: 2007-08-01

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

Secondary effluent of a domestic sewage treatment plant.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2.9 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 22 +/- 2°C
- Aeration of dilution water: aeration of mineral medium plus inoculum (filtrate) for 4 days
- Suspended solids concentration: separation of coarse particles by filtration
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks: 10

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

7 d

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

14 d

% Degradation 3

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

21 d

% Degradation 4

Key result

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference substance has reached the level for ready biodegradability by 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Within 28 days, a degradation of 1 % was determined for Bayticol P acid.
Bayticol P acid is considered to be "Not Readily Biodegradable".

All validity criteria of the test method were met:
The reference substance has reached the level for ready biodegradability by 14 days.
No toxicity of the test item was observed in the toxicity control.
The difference of extremes of replicate values of the removal of test chemical at the end of the test is less than 20 %.
Oxygen depletion in the inoculum blank did not exceed 1.5 mg/L dissolved oxygen after 28 days.