Molybdenum trioxide, reaction products with bis[O,O-bis(2-ethylhexyl)] hydrogen dithiophosphate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 September 1965 to 16 December 1965

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1965

Report date:

1965

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Regulations (Federal Register, August 12, 1961 et seq) under the Federal Hazardous Substances Labeling Act

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']dioxodi-.mu.-thioxodi-, (Mo-Mo)

EC Number:

615-708-0

Cas Number:

72030-25-2

Molecular formula:

N/A

IUPAC Name:

Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']dioxodi-.mu.-thioxodi-, (Mo-Mo)

Constituent 2

Reference substance name:

Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']-.mu.-oxodioxo-.mu.-thioxodi-

Cas Number:

153128-45-1

Molecular formula:

N/A

IUPAC Name:

Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']-.mu.-oxodioxo-.mu.-thioxodi-

Constituent 3

Reference substance name:

Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']oxodi-.mu.-thioxothioxodi-

Molecular formula:

N/A

IUPAC Name:

Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']oxodi-.mu.-thioxothioxodi-

Constituent 4

Reference substance name:

Unspecified 2-ethylhexyl thiophosphate esters

Molecular formula:

N/A

IUPAC Name:

Unspecified 2-ethylhexyl thiophosphate esters

Constituent 5

Reference substance name:

Unspecified 2-ethylhexyl phosphate esters

Molecular formula:

N/A

IUPAC Name:

Unspecified 2-ethylhexyl phosphate esters

Constituent 6

Reference substance name:

Unspecified 2-ethylhexyl dithiophosphate esters

Molecular formula:

N/A

IUPAC Name:

Unspecified 2-ethylhexyl dithiophosphate esters

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

other: albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2211- 2994 grams
- Housing: Individual metal cages elevated from droppings.
- Diet (e.g. ad libitum): Food, consisting of Purina Rabbit Pellets.
- Water (e.g. ad libitum): Water available at all times.

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Type of wrap if used: Rubber dental damming which had been previously wrappred around the trunk of the animal.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): Sample remaining on the skin was removed by gently sponging with a moistened towel.
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amount(s) applied (volume or weight with unit):undiluted sample was applied at dosage levels of 1.00, 2.15, 4.64, and 10.0 ml/kg body weight.

Duration of exposure:

24 hours

Doses:

1.00, 2.15, 4.64, and 10.0 ml/kg of body weight

No. of animals per sex per dose:

4 Groups - composed of 4 albino rabbits.

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Frequent intervals during the 14 day period.
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

10 mL/kg bw

Based on:

test mat.

Mortality:

Two deaths were noted at dose 10.0 ml/kg, one on day 13, and one on day 14.

Clinical signs:

other: At dose levels 1.00 and 2.15 mg/kg the rabbits exhibited normal appearance and behavior throughout the 15 day experimental period. At 4.64 ml/kg level, two rabbits appeared grossly normal and one rabbit appeared slightly emaciated at the termination of th

Gross pathology:

Animals surviving until termination of the study showed no significant gross pathological findings. Gross autopsy findings in the two animals at the 10.0 mg/kg dosage level that died included congestion of the kidney cortices, or cortico-medullary junction irritation, distention of the small intestine with gas, firm pale liver, stomach moderately distended with matted fur, uterus congested, and body fat stores depleted.

Other findings:

Removal of the binders at the end of the 24 hour exposure period all dose levels were stained an intense green color. Staining persisted throughout the observation period.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The acute dermal LD50 for albino rabbits was found to be in the range of 10.0 ml/kg of body weight. The acute toxicity was evaluated using the procedures oulined in the Regulations under the Federal Hazardous Substances Labeling Act.

Executive summary:

The acute dermal LD50 for albino rabbits was found to be in the range of 10.0 ml/kg of body weight. The acute toxicity was evaluated using the procedures oulined in the Regulations under the United States Federal Hazardous Substances Labeling Act.