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EC number: 947-946-9
CAS number: -
in rangefinding test
Cumulative immobilised Daphnia magna
(initial population: 5 Daphnia per replicate)
Table 2: Analysis
of test media during the definitive test
Measured Concentration* (mg/L)
Based on quantitation of dithiophosphate fraction (without molybdenum)
= Limit of quantification (0.01 mg/L)
Table 3: Immobilization
in definitive test
Nominal loading rate (mg/L)
Number ofDaphnia magnaexposed
% Immobilityat 24 hours
% Immobilityat 48 hours
study was conducted in accordance with the requirements of OECD
Chemicals Testing Guideline No. 202. Test
solutions were prepared as Water Accommodated Fractions (WAFs) based on
the recommendations of OECD Series on Testing and Assessment, No. 23. Based
on the results of a range-finding test the definitive test was conducted
at nominal loading rates of 1.0, 3.2, 10, 32 and 100 mg/L under static
conditions, with a concurrent control group.
the start of the test, five juvenile Daphnia magna were added to each
test vessel. The Daphnia magna in each test vessel were observed for
mobility at 24 and 48 hours. The Daphnia magna were not fed during the
of test media were analyzed at0
(fresh media) and 48 hours (old media) usinghigh
performance liquid chromatography (HPLC) with time of flight mass
spectrometry (TOF) based
on quantitation of the dithiophosphatefraction
(without molybdenum) of
the test substance. Analysis
of the test media confirmed measured concentrations to range from 0.0262
to 0.769 mg/L at 0 hours and from 0.0299 to 0.237 mg/L at 48 hours.
Given that the test substance is a multi-constituent substance and
analytical monitoring was limited to the
(without molybdenum) of
the test substance,
the effect concentrations were based on nominal loading rates.
immobilization was observed over the 48-hour exposure period.
The 48-hour EL50 value was therefore determined to be >100 mg/L loading
rate WAF and the No Observed Effect Loading rate (NOEL) was determined
to be 100 mg/L loading rate WAF. The test showed no toxicity at the
limit of solubility of the test substance in the test media.
validity criteria for control immobility (≤10%) and dissolved oxygen (≥3
mg/L) were both satisfied. The test is therefore considered valid.
an OECD 202 study, conducted according to GLP, the acute toxicity of the
test item to the freshwater invertebrate Daphnia magna was investigated
under static conditions and the 48 -Hour EC50 value was determined to be
greater than 100 mg/L loading rate WAF and the No Observed Effect
Loading rate (NOEL) was determined to be 100 mg/L loading rate WAF
(Smithers Viscient (ESG) Ltd, ****).
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