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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28 September 1965 to 16 December 1965
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1965
Report date:
1965

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Regulations (Federal Register, August 12, 1961 et seq) under the Federal Hazardous Substances Labeling Act
Deviations:
not specified
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Reference substance name:
Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']dioxodi-.mu.-thioxodi-, (Mo-Mo)
EC Number:
615-708-0
Cas Number:
72030-25-2
Molecular formula:
N/A
IUPAC Name:
Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']dioxodi-.mu.-thioxodi-, (Mo-Mo)
Constituent 2
Reference substance name:
Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']-.mu.-oxodioxo-.mu.-thioxodi-
Cas Number:
153128-45-1
Molecular formula:
N/A
IUPAC Name:
Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']-.mu.-oxodioxo-.mu.-thioxodi-
Constituent 3
Reference substance name:
Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']oxodi-.mu.-thioxothioxodi-
Molecular formula:
N/A
IUPAC Name:
Molybdenum, bis[O,O-bis(2-ethylhexyl) phosphorodithioato-.kappa.S,.kappa.S']oxodi-.mu.-thioxothioxodi-
Constituent 4
Reference substance name:
Unspecified 2-ethylhexyl thiophosphate esters
Molecular formula:
N/A
IUPAC Name:
Unspecified 2-ethylhexyl thiophosphate esters
Constituent 5
Reference substance name:
Unspecified 2-ethylhexyl phosphate esters
Molecular formula:
N/A
IUPAC Name:
Unspecified 2-ethylhexyl phosphate esters
Constituent 6
Reference substance name:
Unspecified 2-ethylhexyl dithiophosphate esters
Molecular formula:
N/A
IUPAC Name:
Unspecified 2-ethylhexyl dithiophosphate esters
Test material form:
liquid

Test animals

Species:
rat
Strain:
other: Holtzman
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 270-281 grams
- Fasting period before study: Yes. 18 hours before prior to dosage.
- Housing: Wire mesh cages in groups.
- Diet (e.g. ad libitum): food consisting of commercial pellets ad libitum.
- Water (e.g. ad libitum): Water available ad libitum.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Doses:
0.464, 1.00, 2.15, 4.64, 10.0 and 21.5 ml/kg of body weight
No. of animals per sex per dose:
5 males per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: All animals were observed closely day of dosage and at least once daily there after.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight.
Statistics:
Statistical analysis of the mortality data was by the moving average method (Weil, C.S., Biometrics 8, 249, 1952)

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
6.81 mL/kg bw
Based on:
test mat.
Mortality:
Deaths were noted after 24 hours of doses 10.0 and 21.5 ml/kg.
Clinical signs:
Signs noted included depression, mucoid diarrhea with greenish stools. Depressed or absent righting and placement reflexes were observed in these animals which died during the night following the dosage.
Body weight:
All animals showed expected gains in body weight over the observation period.
Gross pathology:
Findings in animals that died included congested lungs and kidneys; stomach filled with green fluid; and small and large intestines distended with green fluid and gas. Gross autopsies performed on the surviving rats at termination showed no significant gross pathological changes.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
The acute oral LD50 for male abino rats was found to be 6.81 ml/kg of body weight. The acute toxicity was evaluated using the procedures outlined in the Regulations under the Federal Hazardous Substances Labeling Act.
Executive summary:

The acute oral LD50 for male abino rats was found to be 6.81 ml/kg of body weight. The acute toxicity was evaluated using the procedures outlined in the Regulations under the Federal Hazardous Substances Labeling Act.